PMID- 28742117
OWN - NLM
STAT- MEDLINE
DCOM- 20170925
LR  - 20201214
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Comparison of GCaMP3 and GCaMP6f for studying astrocyte Ca2+ dynamics in the 
      awake mouse brain.
PG  - e0181113
LID - 10.1371/journal.pone.0181113 [doi]
LID - e0181113
AB  - In recent years it has become increasingly clear that astrocytes play a much more 
      active role in neural processes than the traditional view of them as supporting 
      cells suggests. Although not electrically excitable, astrocytes exhibit diverse 
      Ca2+ dynamics across spatial and temporal scales, more or less dependent on the 
      animal's behavioral state. Ca2+ dynamics range from global elevations lasting 
      multiple seconds encompassing the soma up to the finest processes, to short 
      elevations restricted to so-called microdomains within fine processes. 
      Investigations of astrocyte Ca2+ dynamics have particularly benefitted from the 
      development of Genetically-Encoded Calcium Indicators (GECIs). GECI expression 
      can be achieved non-invasively in a cell type-specific manner and it can be 
      genetically targeted to subcellular domains. The GCaMP family, a group of GECIs 
      derived from the green fluorescent protein, has experienced some of the fastest 
      advancements during the past decade. As a consequence we are now facing the 
      challenge of needing to compare published data obtained with different versions 
      of GECIs. With the intention to provide some guidance, here we compared Ca2+ 
      dynamics across scales in awake transgenic mice expressing either the 
      well-established GCaMP3, or the increasingly popular GCaMP6f, specifically in 
      astrocytes. We found that locomotion-induced global Ca2+ elevations in cortical 
      astrocytes displayed only minor kinetic differences and their apparent dynamic 
      ranges for Ca2+ sensing were not different. In contrast, Ca2+ waves in processes 
      and microdomain Ca2+ transients were much more readily detectable with GCaMP6f. 
      Our findings suggest that behavioral state-dependent global astrocyte Ca2+ 
      responses can be studied with either GCaMP3 or GCaMP6f whereas the latter is more 
      appropriate for studies of spatially restricted weak and fast Ca2+ dynamics.
FAU - Ye, Liang
AU  - Ye L
AD  - Department of Cellular and Integrative Physiology, University of Texas Health San 
      Antonio, San Antonio, TX, United States of America.
AD  - Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
FAU - Haroon, Mateen A
AU  - Haroon MA
AD  - Department of Cellular and Integrative Physiology, University of Texas Health San 
      Antonio, San Antonio, TX, United States of America.
FAU - Salinas, Angelica
AU  - Salinas A
AD  - Department of Cellular and Integrative Physiology, University of Texas Health San 
      Antonio, San Antonio, TX, United States of America.
AD  - Center for Biomedical Neuroscience, University of Texas Health San Antonio, San 
      Antonio, TX, United States of America.
FAU - Paukert, Martin
AU  - Paukert M
AUID- ORCID: 0000-0001-5323-3164
AD  - Department of Cellular and Integrative Physiology, University of Texas Health San 
      Antonio, San Antonio, TX, United States of America.
AD  - Center for Biomedical Neuroscience, University of Texas Health San Antonio, San 
      Antonio, TX, United States of America.
AD  - Biomedical Engineering Joint Graduate Program, UTSA/UTHSA, San Antonio, TX, 
      United States of America.
LA  - eng
GR  - R03 AA022239/AA/NIAAA NIH HHS/United States
GR  - R25 GM095480/GM/NIGMS NIH HHS/United States
GR  - T32 NS082145/NS/NINDS NIH HHS/United States
GR  - R01 AA025128/AA/NIAAA NIH HHS/United States
GR  - R01 MH113780/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20170724
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Recombinant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Brain/*metabolism
MH  - Calcium Signaling/*physiology
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Kinetics
MH  - Locomotion/physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Fluorescence, Multiphoton
MH  - Recombinant Proteins/genetics/metabolism
PMC - PMC5524333
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/26 06:00
MHDA- 2017/09/26 06:00
PMCR- 2017/07/24
CRDT- 2017/07/26 06:00
PHST- 2017/01/13 00:00 [received]
PHST- 2017/06/25 00:00 [accepted]
PHST- 2017/07/26 06:00 [entrez]
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2017/09/26 06:00 [medline]
PHST- 2017/07/24 00:00 [pmc-release]
AID - PONE-D-17-01736 [pii]
AID - 10.1371/journal.pone.0181113 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 24;12(7):e0181113. doi: 10.1371/journal.pone.0181113. 
      eCollection 2017.