PMID- 28744576
OWN - NLM
STAT- MEDLINE
DCOM- 20171129
LR  - 20180710
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 96
IP  - 10
DP  - 2017 Oct
TI  - Cytogenetics with flow cytometry in lymph node/extranodal tissue biopsies is 
      sensitive to assist the early diagnosis of suspected lymphomas.
PG  - 1673-1680
LID - 10.1007/s00277-017-3066-y [doi]
AB  - Few studies have examined the value of cytogenetic studies with flow cytometry 
      (FC) in lymph node/extranodal tissue biopsies with suspected lymphoma. To 
      evaluate this, G-banded karyotyping and/or fluorescence in situ hybridization 
      (FISH) with FC immunophenotyping were performed on 185 lymph node or extranodal 
      tissue biopsy specimens with suspected lymphoma. Complete cytogenetic analysis of 
      lymph node/extranodal tissue was successful in 174 cases (94.1%) and 57.5% 
      demonstrated chromosomal abnormalities. In 116 malignant lymphoma cases, 83.8% 
      showed abnormalities. In 74 B cell lymphomas (B-NHL), abnormalities were more 
      frequent in lymph node/extranodal tissues than in bone marrow by conventional 
      cytogenetics (CC, 97.2 vs 26.1%), FISH (70.6 vs 17.6%), and FC (98.6 vs 28.4%). 
      Three B-NHL diagnoses were confirmed by re-biopsy of lymph nodes due to the 
      presence of abnormalities in the first biopsy, but no evidence of malignancy in 
      pathological, FC, or IgH/TCR gene rearrangement analyses. In 29 T cell lymphomas 
      (T-NHL), abnormalities were more frequent in lymph nodes than in bone marrow by 
      CC (67.9 vs 21.4%) and FC (75.9 vs 27.6%) analyses. As expected, in 13 Hodgkin 
      lymphoma cases, abnormalities were more frequent in lymph nodes than bone marrow 
      by CC (41.7 vs 16.7%) and FC (30.8 vs 7.7%) analyses. In 56 reactive lymphoid 
      hyperplasias (RLH), 7.1% had conventional clonal cytogenetic abnormalities. Two 
      of these patients died of disease progression and two had their pathological 
      diagnosis revised after the second review. These findings indicate that 
      cytogenetic analysis combined with FC in lymph node/extranodal tissue biopsies 
      can provide critical information in the auxiliary diagnosis of lymphoma.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Li, Qinlu
AU  - Li Q
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Mao, Xia
AU  - Mao X
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Zhang, Heng
AU  - Zhang H
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Huang, Lifang
AU  - Huang L
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Meng, Fankai
AU  - Meng F
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Wei, Jia
AU  - Wei J
AUID- ORCID: 0000-0002-6435-9368
AD  - Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China. 
      jiawei@tjh.tjmu.edu.cn.
LA  - eng
GR  - 81300390/National Natural Science Foundation of China/
GR  - 81570110/National Natural Science Foundation of China/
GR  - 81500082/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20170725
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Female
MH  - *Flow Cytometry
MH  - *Gene Rearrangement
MH  - *Hodgkin Disease/diagnosis/genetics/pathology
MH  - Humans
MH  - *Lymphoma, B-Cell/diagnosis/genetics/pathology
MH  - *Lymphoma, T-Cell/diagnosis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Sentinel Lymph Node Biopsy
OTO - NOTNLM
OT  - Chromosome abnormalities
OT  - FISH
OT  - Flow cytometry
OT  - Lymphoma
OT  - Reactive lymphoid hyperplasia
EDAT- 2017/07/27 06:00
MHDA- 2017/12/01 06:00
CRDT- 2017/07/27 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/07/05 00:00 [accepted]
PHST- 2017/07/27 06:00 [pubmed]
PHST- 2017/12/01 06:00 [medline]
PHST- 2017/07/27 06:00 [entrez]
AID - 10.1007/s00277-017-3066-y [pii]
AID - 10.1007/s00277-017-3066-y [doi]
PST - ppublish
SO  - Ann Hematol. 2017 Oct;96(10):1673-1680. doi: 10.1007/s00277-017-3066-y. Epub 2017 
      Jul 25.