PMID- 28745079
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20240313
IS  - 2058-7384 (Electronic)
IS  - 0394-6320 (Print)
IS  - 0394-6320 (Linking)
VI  - 30
IP  - 3
DP  - 2017 Sep
TI  - Cytokine production in mammary adenocarcinoma and its microenvironmental cells in 
      patients with or without metastases in regional lymph nodes.
PG  - 308-314
LID - 10.1177/0394632017720055 [doi]
AB  - In recent years, the concept of formation of a sufficiently autonomous cytokine 
      network in a malignant tumour has emerged. In this regard, the data on the role 
      of this network and its signalling pathways in the process of metastasis are an 
      interesting topic. The aim of this study was to evaluate the in vitro 
      cytokine-producing potential of mammary adenocarcinoma (MAC; and cells of its 
      microenvironment) from patients with or without metastases in regional lymph 
      nodes (LNs). By enzyme-linked immunosorbent assays of culture supernatants, we 
      studied the cytokine production by biopsy samples of MAC: spontaneous and 
      stimulated by polyclonal activators (PAs: phytohaemagglutinin, concanavalin A and 
      lipopolysaccharide). The levels of spontaneous production of interleukin (IL)-10 
      and granulocyte colony-stimulating factor (G-CSF) and the amounts of IL-2, IL-10, 
      G-CSF and monocyte chemoattractant protein-1 (MCP-1) produced during stimulation 
      by PAs, as well as the index of stimulation by polyclonal activators (ISPA) for 
      IL-2 production, were lower for MAC with LN metastasis than for MAC without LN 
      metastasis. The levels of spontaneous production of IL-2 and interferon (IFN)-gamma 
      and the ISPA for granulocyte-macrophage colony-stimulating factor (GM-CSF) 
      production were higher for MAC with LN metastasis. There were only three pairwise 
      correlations between the produced cytokines that were specific to MAC with LN 
      metastasis: IL-2 and IFN-gamma, IL-6 and GM-CSF, and IL-8 and GM-CSF. There were 10 
      pairwise correlations between the produced cytokines that were specific to 
      nonmetastasising MAC: IL-6 and IL-10, IL-6 and MCP-1, IL-8 and IL-10, IL-8 and 
      MCP-1, IL-10 and G-CSF, IL-10 and MCP-1, IFN-gamma and MCP-1, MCP-1 and G-CSF, G-CSF 
      and IL-1Ra, and GM-CSF and tumour necrosis factor (TNF)-alpha. Our data indicate that 
      metastatic tumours show desynchronisation of many pathways of induction and 
      synthesis of cytokines that are characteristic of nonmetastatic tumours.
FAU - Autenshlyus, Alexander
AU  - Autenshlyus A
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
AD  - 2 Institute of Molecular Biology and Biophysics, Novosibirsk, Russia.
FAU - Arkhipov, Sergey
AU  - Arkhipov S
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
AD  - 2 Institute of Molecular Biology and Biophysics, Novosibirsk, Russia.
FAU - Mikhailova, Elena
AU  - Mikhailova E
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
AD  - 2 Institute of Molecular Biology and Biophysics, Novosibirsk, Russia.
FAU - Marinkin, Igor
AU  - Marinkin I
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
FAU - Arkhipova, Valentina
AU  - Arkhipova V
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
FAU - Mogilnaya, Ekaterina
AU  - Mogilnaya E
AD  - 1 Novosibirsk State Medical University, Novosibirsk, Russia.
FAU - Varaksin, Nikolay
AU  - Varaksin N
AD  - 3 Joint-Stock Company Vector-Best, Novosibirsk, Russia.
FAU - Rukavishnikov, Michael
AU  - Rukavishnikov M
AD  - 3 Joint-Stock Company Vector-Best, Novosibirsk, Russia.
LA  - eng
PT  - Journal Article
DEP - 20170726
PL  - England
TA  - Int J Immunopathol Pharmacol
JT  - International journal of immunopathology and pharmacology
JID - 8911335
RN  - 0 (Cytokines)
SB  - IM
MH  - Adenocarcinoma/*metabolism/pathology
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Middle Aged
MH  - Sentinel Lymph Node/pathology
MH  - Tumor Microenvironment
PMC - PMC5815260
OTO - NOTNLM
OT  - cytokine
OT  - cytokine-producing potential
OT  - mammary adenocarcinoma
OT  - metastasis into regional lymph nodes
OT  - polyclonal activators
COIS- Declaration of conflicting interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship and/or publication 
      of this article.
EDAT- 2017/07/27 06:00
MHDA- 2018/05/23 06:00
PMCR- 2017/09/01
CRDT- 2017/07/27 06:00
PHST- 2017/07/27 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/07/27 06:00 [entrez]
PHST- 2017/09/01 00:00 [pmc-release]
AID - 10.1177_0394632017720055 [pii]
AID - 10.1177/0394632017720055 [doi]
PST - ppublish
SO  - Int J Immunopathol Pharmacol. 2017 Sep;30(3):308-314. doi: 
      10.1177/0394632017720055. Epub 2017 Jul 26.