PMID- 2874682
OWN - NLM
STAT- MEDLINE
DCOM- 19860917
LR  - 20171116
IS  - 0065-1281 (Print)
IS  - 0065-1281 (Linking)
VI  - 79
IP  - 1
DP  - 1986
TI  - Enzyme patterns in human endocytotic multinucleate giant cells--a histochemical 
      study.
PG  - 1-10
AB  - A series of human multinucleate giant cells (MGCs) of the endocytotic type were 
      studied using enzyme histochemical methods for dehydrogenases, glycosidases, 
      phosphatases, and peptidases. Several enzyme patterns were found. The subgroup of 
      MGCs associated with inflammatory granulomatous processes (sarcoidosis, 
      granulomatous myositis, familial granulomatosis, lymphogranuloma, granulomatous 
      cholangitis) was characterized by high activities of nonspecific esterase (NE) 
      and tartrate-sensitive acid phosphatase (AcPase-Ts). There was no detectable 
      activity of peptidases or tartrate-resistant isoenzyme of acid phosphatase 
      (AcPase-Tr). This enzyme equipment was indistinguishable from that in mononuclear 
      precursors in the granulomas. The other MGCs of the series displayed enzyme 
      patterns substantially different from their monocytic precursors (blood monocytes 
      and Langerhans cells). The subgroup of foreign body associated MGCs (resorption 
      of fat, keratin, and suture material) was characterized by high activities of NE, 
      AcPase-Tr, and greatly variable activities of both peptidases studied. The latter 
      lacked predilection for certain subcellular regions. The subgroup of osteoclasts 
      and so-called giant cell tumours (osteoclastoma, giant cell tumour of soft parts, 
      giant cell epulis of peripheral, and central types) displayed very low activity 
      of NE, high activity of AcPase-Tr, and strong activities of peptidases. The 
      latter were localized near the surface membrane of the polykarya. MGCs in 
      histiocytosis X (HX) differed from the previous group by higher values of NE in 
      average. All MGC types had common denominator in the absence of alkaline 
      phosphatase activity, on average intense dehydrogenase activities, mostly low 
      beta-glucuronidase and highly variable alpha-mannosidase activities. The enzyme 
      pattern heterogeneity is discussed with regard to the phenomenon of enzyme 
      induction and depression occurring in course of polykaryon production. The 
      variability of phenomenon may reflect reactive adaptation to varying functional 
      demands imposed on MGCs under different conditions.
FAU - Elleder, M
AU  - Elleder M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Isoenzymes)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.1 (Carboxylesterase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.4.11.- (Aminopeptidases)
RN  - EC 3.4.11.2 (CD13 Antigens)
RN  - EC 3.4.14.- (Dipeptidyl-Peptidases and Tripeptidyl-Peptidases)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
SB  - IM
MH  - Acid Phosphatase/*metabolism
MH  - Aminopeptidases/*metabolism
MH  - CD13 Antigens
MH  - Carboxylesterase
MH  - Carboxylic Ester Hydrolases/*metabolism
MH  - Carcinoma/*pathology
MH  - Dipeptidyl Peptidase 4
MH  - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/*metabolism
MH  - Endocytosis
MH  - Granuloma/*enzymology/pathology
MH  - Humans
MH  - Inflammation
MH  - Isoenzymes/metabolism
MH  - Osteoclasts/cytology/*enzymology
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - S0065-1281(86)80091-5 [pii]
AID - 10.1016/S0065-1281(86)80091-5 [doi]
PST - ppublish
SO  - Acta Histochem. 1986;79(1):1-10. doi: 10.1016/S0065-1281(86)80091-5.