PMID- 2875086
OWN - NLM
STAT- MEDLINE
DCOM- 19861021
LR  - 20071114
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 250
IP  - 3
DP  - 1986 Aug 15
TI  - LHRH and catecholamine neuronal systems in the olfactory bulb of the mouse.
PG  - 352-63
AB  - The luteinizing-hormone-releasing hormone (LHRH) components of the mouse 
      olfactory system were studied by using the indirect immunohistofluorescence 
      technique. LHRH-positive cell bodies were found in both the main olfactory bulb 
      (MOB) and the accessory olfactory bulb (AOB); two distinct cell groups were 
      found--one located adjacent to the AOB in the dorsal part of the MOB and a second 
      in the superficial ventromedial aspect of the MOB. LHRH-positive fibers were 
      found predominantly in the posterior half of the main olfactory bulb innervating 
      the olfactory nerve layer, the glomerular layer, the external plexiform layer, 
      and the mitral cell layer, and small numbers innervated the AOB. The greatest 
      density of LHRH-immunoreactive fibers was seen adjacent to the AOB where one 
      group of LHRH-positive cells was noted. Sections adjacent to those stained with 
      LHRH antibody were analysed for tyrosine hydroxylase (TH) and dopamine 
      beta-hydroxylase (DBH). The TH staining was intense for both cells and fibers in 
      the glomerular layer of the MOB. In the AOB lower numbers of cell bodies and 
      fibers were seen in the glomerular layer. The internal granular layer and 
      internal plexiform layers were both stained, the internal granular layer showing 
      more intensely fluorescent fibers with a higher density. Since no overlapping 
      DBH-positive fibers were found here, these TH-positive nerve endings may be 
      dopaminergic. DBH staining was confined to sparse networks of weakly fluorescent 
      fibers with the highest numbers in the internal plexiform layer of the AOB and 
      fewer fibers in the external plexiform, rostral, and granular layers of the MOB. 
      Elution-restaining experiments revealed that the LHRH-positive and TH-positive 
      elements represent different cell populations.
FAU - Rosser, A E
AU  - Rosser AE
FAU - Hokfelt, T
AU  - Hokfelt T
FAU - Goldstein, M
AU  - Goldstein M
LA  - eng
GR  - NIMH 02714/MH/NIMH NIH HHS/United States
GR  - NINCDS 06801/DS/DS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
SB  - IM
MH  - Animals
MH  - Dopamine beta-Hydroxylase/*metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - Mice
MH  - Olfactory Bulb/*metabolism
MH  - Tyrosine 3-Monooxygenase/*metabolism
EDAT- 1986/08/15 00:00
MHDA- 1986/08/15 00:01
CRDT- 1986/08/15 00:00
PHST- 1986/08/15 00:00 [pubmed]
PHST- 1986/08/15 00:01 [medline]
PHST- 1986/08/15 00:00 [entrez]
AID - 10.1002/cne.902500308 [doi]
PST - ppublish
SO  - J Comp Neurol. 1986 Aug 15;250(3):352-63. doi: 10.1002/cne.902500308.