PMID- 28751109
OWN - NLM
STAT- MEDLINE
DCOM- 20180124
LR  - 20181010
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 93
DP  - 2018 Jan
TI  - Polymorphism and peptide-binding specificities of porcine major 
      histocompatibility complex (MHC) class I molecules.
PG  - 236-245
LID - S0161-5890(17)30196-7 [pii]
LID - 10.1016/j.molimm.2017.06.024 [doi]
AB  - The swine lymphocyte antigen class I (SLA I) is a highly polymorphic gene 
      superfamily that plays an important role in swine anti-viral immune responses. 
      However, an understanding of the highly variable sites and peptide-binding 
      specificities of SLA I molecule is limited. In this study, a total of 27 SLA I 
      alleles were identified from 3 Tibetan wild boars and 3 Heishan pigs. The 
      phylogenetic relationship between the Tibetan wild boar and other breeds was 
      analyzed using bioinformatics methods, and the highly variable sites were noted 
      in the three dimensional structures of SLA I. Peptides from the porcine 
      reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) 
      were screened with a bioinformatic method and refolding assay in vitro. The 
      superior SLA I molecules, which have the ability to combine with more peptides, 
      were selected from the Tibetan wild boars and Heishan pigs. The results showed 
      that the SLA I of the Tibetan wild boars was not divergent from other pig breeds 
      and that high-variation sites were mostly located in the peptide binding groove 
      (PBG), suggesting that high variation sites could determines the peptide-binding 
      characteristics and would possibly influences peptide-specific CD8(+) T cell 
      recognition. The SLA I allele SLA-1*0302 (known as KY113114) of the Tibetan wild 
      boar formed stable complexes with three PRRSV peptides, and the SLA-3*hs0202 
      (KJ555032) from Heishan pigs was able to bind with four IAV peptides. The results 
      from this study may benefit vaccine development and may help control IAV and 
      PRRSV in swine.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Fan, Shuhua
AU  - Fan S
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China; College of Life Science and Agronomy, 
      Zhoukou Normal University, Zhoukou, PR China.
FAU - Wang, Yongli
AU  - Wang Y
AD  - College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, PR 
      China.
FAU - Wang, Song
AU  - Wang S
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China.
FAU - Wang, Xian
AU  - Wang X
AD  - College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, PR 
      China.
FAU - Wu, Yanan
AU  - Wu Y
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China.
FAU - Li, Zibin
AU  - Li Z
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China.
FAU - Zhang, Nianzhi
AU  - Zhang N
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China. Electronic address: 
      zhangnianzhi@cau.edu.cn.
FAU - Xia, Chun
AU  - Xia C
AD  - Department of Microbiology and Immunology, College of Veterinary Medicine, China 
      Agricultural University, Beijing, PR China; Key Laboratory of Animal Epidemiology 
      and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, PR 
      China. Electronic address: xiachun@cau.edu.cn.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170724
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Antigens, Viral)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (swine leukocyte antigen)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Animals, Domestic/genetics/immunology
MH  - Animals, Wild/genetics/immunology
MH  - Antigens, Viral/immunology
MH  - Breeding
MH  - Disease Resistance
MH  - *Genes, MHC Class I
MH  - Histocompatibility Antigens Class I/chemistry/genetics/*metabolism
MH  - Influenza A virus/immunology
MH  - Peptides/immunology
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Protein Folding
MH  - Species Specificity
MH  - Substrate Specificity
MH  - Sus scrofa/immunology
MH  - Swine/genetics/*immunology
MH  - Swine Diseases/genetics
MH  - Tibet
MH  - Viral Proteins/immunology
MH  - Viral Vaccines
OTO - NOTNLM
OT  - CTL immunity
OT  - MHC I
OT  - Peptide
OT  - Polymorphism
OT  - Tibetan wild boar
EDAT- 2017/07/29 06:00
MHDA- 2018/01/25 06:00
CRDT- 2017/07/29 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/05/07 00:00 [revised]
PHST- 2017/06/10 00:00 [accepted]
PHST- 2017/07/29 06:00 [pubmed]
PHST- 2018/01/25 06:00 [medline]
PHST- 2017/07/29 06:00 [entrez]
AID - S0161-5890(17)30196-7 [pii]
AID - 10.1016/j.molimm.2017.06.024 [doi]
PST - ppublish
SO  - Mol Immunol. 2018 Jan;93:236-245. doi: 10.1016/j.molimm.2017.06.024. Epub 2017 
      Jul 24.