PMID- 28752701
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20230805
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 46
IP  - 2
DP  - 2017 Mar 25
TI  - [Effects of myeloid specific deficiency of FBXW7 on lung metastasis of murine 
      melanoma].
PG  - 111-117
AB  - Objective: To investigate the effects of myeloid-specific deficiency of FBXW7 on 
      lung metastasis of murine B16F10 melanoma and its mechanisms. Methods: Mice 
      carrying the floxed allele of FBXW7 and lysozyme M-Cre were used for generation 
      of mice with myeloid cell-specific deletion of FBXW7. Mouse genotypes were 
      examined by genomic DNA PCR. B16F10 cells in PBS were injected into the tail vein 
      of Lysm(-)FBXW7(f/f) and Lysm(+)FBXW7 (f/f) mice. After 14 d, the mice were 
      sacrificed, and the lungs were removed and weighed. B16F10 tumor colonies in the 
      lungs were counted. The myeloid cells were analyzed by flow cytometry. Results: 
      Myeloid-specific deficiency of FBXW7 mice were generated successfully, as FBXW7 
      expressions in peritoneal macrophages and bone marrow-derived macrophages (BMDMs) 
      of Lysm(+)FBXW7(f/f) mice were knockdown. Flow cytometry results showed the 
      deletion of FBXW7 in myeloid lineages did not affect the development of myeloid 
      immune cell subsets. Metastasis was reduced in Lysm(+)FBXW7 (f/f) mice compared 
      with control mice. The number of tumor colonies was 165+/-42, 122+/-12 respectively. 
      The proportion of metastasis-associated macrophages (MAM) in the lungs of 
      Lysm(+)FBXW7 (f/f) mice was reduced [(23.15+/-7.59)% vs (13.13+/-2.26)%], while the 
      proportion of resident macrophages was increased [(5.426+/-0.42)% vs 
      (10.42+/-1.90)%]. The proportion of myeloid-derived suppressor cells in the lung 
      showed no difference between Lysm(-)FBXW7(f/f) and Lysm(+)FBXW7 (f/f) mice. 
      Conclusion: Myeloid-specific deficiency of FBXW7 can inhibit lung metastasis of 
      B16F10 melanoma in mice, and the mechanism may be associated with regulation of 
      MAM in the metastatic tumor lesions.
FAU - Wei, Zhang
AU  - Wei Z
AD  - Department of Oncology, Hangzhou First People's Hospital, Hangzhou 310006, China; 
      Department of Chemotherapy, Hangzhou cancer Hospltal, Hangzhou 310002, China; 
      Institute of Immunology, Zhejiang University, Hangzhou 310058, China.
FAU - Lihua, Lai
AU  - Lihua L
AD  - Institute of Immunology, Zhejiang University, Hangzhou 310058, China.
FAU - Qingqing, Wang
AU  - Qingqing W
AD  - Institute of Immunology, Zhejiang University, Hangzhou 310058, China. 
      wqq@zju.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (F-Box-WD Repeat-Containing Protein 7)
RN  - 0 (Fbxw7 protein, mouse)
SB  - IM
MH  - Animals
MH  - *F-Box-WD Repeat-Containing Protein 7/deficiency/genetics
MH  - Gene Deletion
MH  - Gene Expression Regulation, Neoplastic
MH  - Lung Neoplasms/enzymology/*secondary
MH  - *Macrophages/enzymology
MH  - *Melanoma/enzymology/genetics
MH  - Mice
MH  - Myeloid Cells/enzymology
PMC - PMC10396932
EDAT- 2017/07/29 06:00
MHDA- 2019/05/14 06:00
PMCR- 2017/04/25
CRDT- 2017/07/29 06:00
PHST- 2017/07/29 06:00 [entrez]
PHST- 2017/07/29 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2017/04/25 00:00 [pmc-release]
AID - zjdxxbyxb-46-2-111 [pii]
AID - 10.3785/j.issn.1008-9292.2017.04.01 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2017 Mar 25;46(2):111-117. doi: 
      10.3785/j.issn.1008-9292.2017.04.01.