PMID- 28755228
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR  - 20180723
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1638
DP  - 2017
TI  - Date Palm Sex Differentiation Based on Fluorescence In Situ Hybridization (FISH).
PG  - 245-256
LID - 10.1007/978-1-4939-7159-6_20 [doi]
AB  - In situ hybridization (ISH) is used to visualize defined DNA sequences in 
      cellular preparations by hybridization of complementary probe sequences. 
      Recently, the fluorescence in situ hybridization (FISH) technique has become a 
      powerful and useful tool for the direct detection of specific DNA fragments in 
      the genome. Ribosomal DNA genes (45S and 5S rDNA) are commonly used as markers 
      for the physical mapping of plant chromosomes to analyze genomic organization. 
      Here we describe cytological-based markers to differentiate date palm gender 
      through localization of 45S and 5S rDNA markers on date palm chromosomes using 
      FISH.
FAU - Atia, Mohamed A M
AU  - Atia MAM
AD  - Molecular Genetics and Genome Mapping Laboratory (MGGM), Agricultural Genetic 
      Engineering Research Institute (AGERI), ARC, Giza, 12619, Egypt. 
      matia@ageri.sci.eg.
FAU - Adawy, Sami S
AU  - Adawy SS
AD  - Molecular Genetics and Genome Mapping Laboratory (MGGM), Agricultural Genetic 
      Engineering Research Institute (AGERI), ARC, Giza, 12619, Egypt.
FAU - El-Itriby, Hanaiya A
AU  - El-Itriby HA
AD  - National Gene Bank, Agriculture Research Center, Giza, Egypt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Genome, Plant/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Phoeniceae/*genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Sex Differentiation/*genetics
OTO - NOTNLM
OT  - Cytogenetic marker
OT  - FISH
OT  - Molecular markers
OT  - Sex determination
OT  - rDNA
EDAT- 2017/07/30 06:00
MHDA- 2018/04/18 06:00
CRDT- 2017/07/30 06:00
PHST- 2017/07/30 06:00 [entrez]
PHST- 2017/07/30 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
AID - 10.1007/978-1-4939-7159-6_20 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1638:245-256. doi: 10.1007/978-1-4939-7159-6_20.