PMID- 28757007
OWN - NLM
STAT- MEDLINE
DCOM- 20180413
LR  - 20180413
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 207
DP  - 2017 Aug
TI  - Different susceptibility and pathogenesis of rabbit genotype 3 hepatitis E virus 
      (HEV-3) and human HEV-3 (JRC-HE3) in SPF rabbits.
PG  - 1-6
LID - S0378-1135(17)30471-6 [pii]
LID - 10.1016/j.vetmic.2017.05.019 [doi]
AB  - Hepatitis E virus (HEV) is an increasingly important zoonotic infection in humans 
      with HEV genotypes 3 and 4 being recognized as zoonotic pathogens. The relatively 
      recently isolated genotype 3 rabbit HEV (rHEV-3) and the more well known genotype 
      3 isolates from humans and swine (hsHEV-3) have all been confirmed experimentally 
      to be capable of infecting both non-human primates and specific-pathogen free 
      (SPF) pigs. In a previous study rHEV-3 was shown to cause acute hepatitis in 
      experimentally infected rabbits. However, whether hsHEV-3 can productively infect 
      rabbits remained unclear. The objective of this study was to investigate the 
      experimental infection of rabbits with human HEV-3 (hHEV-3, JRC-HE3), to compare 
      it to that with rHEV-3 (CHN-BJ-rb14) and to further characterise the pathogenesis 
      of the two isolates. All animals inoculated with rHEV-3 (CHN-BJ-rb14) became 
      infected, exhibiting an intermittent viremia, elevated liver enzymes, and 
      persistent fecal virus shedding throughout the 15 week study period. Liver 
      histopathology showed acute inflammation and both positive- and negative-stranded 
      viral RNA was detected in various tissues from necropsied rabbits. By contrast, 
      neither sero-conversion nor alanine aminotransferase (ALT) elevation was observed 
      in most rabbits inoculated with hHEV-3 (JRC-HE3). In addition, rHEV-3 
      (CHN-BJ-rb14) but not hHEV-3 (JRC-HE3) recovered from primary infected rabbits 
      was transmissible to naive rabbits. These results showed that SPF rabbits are 
      readily susceptible to infection with rHEV-3 (CHN-BJ-rb14) but not hHEV-3 
      (JRC-HE3), which might indicate the influence of viral genomic organization on 
      its pathogenicity.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Zhang, Yulin
AU  - Zhang Y
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
FAU - Gong, Wanyun
AU  - Gong W
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
FAU - Song, William Tianshi
AU  - Song WT
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
FAU - Fu, Hongwei
AU  - Fu H
AD  - School of Laboratory Medicine, Tianjin Medical University, Tianjin, 300203, 
      China.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
FAU - Li, Manyu
AU  - Li M
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
FAU - Wang, Ling
AU  - Wang L
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China. 
      Electronic address: lingwang@bjmu.edu.cn.
FAU - Zhuang, Hui
AU  - Zhuang H
AD  - Department of Microbiology and Center of Infectious Disease, School of Basic 
      Medical Sciences, Peking University Health Science Center, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20170529
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Disease Susceptibility/*veterinary
MH  - Hepatitis E/pathology/*veterinary/virology
MH  - Hepatitis E virus/classification/*genetics/*pathogenicity
MH  - Hepatitis, Viral, Animal/pathology/*virology
MH  - Humans
MH  - Liver/pathology/virology
MH  - Rabbits
MH  - Specific Pathogen-Free Organisms
OTO - NOTNLM
OT  - HEV genotype 3 (HEV-3)
OT  - Infection
OT  - Pathogenesis
OT  - Rabbits
EDAT- 2017/08/02 06:00
MHDA- 2018/04/14 06:00
CRDT- 2017/08/01 06:00
PHST- 2017/04/20 00:00 [received]
PHST- 2017/05/20 00:00 [revised]
PHST- 2017/05/22 00:00 [accepted]
PHST- 2017/08/01 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/04/14 06:00 [medline]
AID - S0378-1135(17)30471-6 [pii]
AID - 10.1016/j.vetmic.2017.05.019 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Aug;207:1-6. doi: 10.1016/j.vetmic.2017.05.019. Epub 2017 May 
      29.