PMID- 28757015
OWN - NLM
STAT- MEDLINE
DCOM- 20180413
LR  - 20180413
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 207
DP  - 2017 Aug
TI  - Dual infections of CD163 expressing NPTr epithelial cells with influenza A virus 
      and PRRSV.
PG  - 143-148
LID - S0378-1135(17)30645-4 [pii]
LID - 10.1016/j.vetmic.2017.06.012 [doi]
AB  - In the pig, respiratory co-infections involving various pathogens are far more 
      frequent than single infections. Amongst respiratory viruses, swine influenza 
      type A virus (swIAV) and porcine reproductive and respiratory syndrome virus 
      (PRRSV) are frequently associated. Previously, we performed co-infections with 
      swIAV and PRRSV in porcine alveolar macrophages (PAM) and precision cut lung 
      slices (PCLS). With these two approaches it was practically impossible to have 
      co-infections of the same cells as the main target cell of swIAV is the 
      epithelial cell while the main target of PRRSV is the PAM. This constraint makes 
      the study of interference between the two viruses difficult at the cellular 
      level. In the current report, an epithelial cell line expressing, CD163, the main 
      receptor of PRRSV was generated. This cell line receptive for both viruses was 
      used to assess the interference between the two viruses. Results showed that 
      swIAV as well as PRRSV, even if they interacted differently with the modified 
      epithelial cells, were clearly interfering with each other regarding their 
      replication when they infected a same cell with consequences within the cellular 
      antiviral response. Our modified cell line, receptive to both viruses, can be 
      used as a tool to assess interference between swIAV and PRRSV in a same cell as 
      it probably happens in the porcine host.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Provost, Chantale
AU  - Provost C
AD  - Swine and Poultry Infectious Diseases Research Center (CRIPA) et Groupe de 
      Recherche sur les Maladies Infectieuses en Production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, Canada.
FAU - Hamonic, Glenn
AU  - Hamonic G
AD  - Vaccine and Infectious Disease Organization (VIDO)-International Vaccine Centre 
      (InterVac) and the Department of Veterinary Microbiology, University of 
      Saskatchewan, Saskatoon, Saskatchewan, Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Swine and Poultry Infectious Diseases Research Center (CRIPA) et Groupe de 
      Recherche sur les Maladies Infectieuses en Production animale (GREMIP), Faculte 
      de medecine veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, Canada.
FAU - Meurens, Francois
AU  - Meurens F
AD  - BIOEPAR, INRA, Oniris, La Chantrerie, 44307, Nantes, France. Electronic address: 
      francois.meurens@oniris-nantes.fr.
LA  - eng
PT  - Journal Article
DEP - 20170616
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - *Antigens, CD
MH  - *Antigens, Differentiation, Myelomonocytic
MH  - Cell Line
MH  - Coinfection
MH  - Dogs
MH  - Epithelial Cells/*metabolism/virology
MH  - Gene Expression Regulation/immunology
MH  - Influenza A virus/*physiology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - *Receptors, Cell Surface
MH  - Swine
MH  - Trachea/cytology
MH  - Virus Cultivation
MH  - Virus Replication
OTO - NOTNLM
OT  - CD163
OT  - Co-infection
OT  - Influenza A virus
OT  - PRRSV
OT  - Pig epithelial cells
EDAT- 2017/08/02 06:00
MHDA- 2018/04/14 06:00
CRDT- 2017/08/01 06:00
PHST- 2017/05/22 00:00 [received]
PHST- 2017/06/14 00:00 [revised]
PHST- 2017/06/15 00:00 [accepted]
PHST- 2017/08/01 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/04/14 06:00 [medline]
AID - S0378-1135(17)30645-4 [pii]
AID - 10.1016/j.vetmic.2017.06.012 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Aug;207:143-148. doi: 10.1016/j.vetmic.2017.06.012. Epub 2017 
      Jun 16.