PMID- 28757162
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20181113
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 9
IP  - 2
DP  - 2017 Aug 8
TI  - An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal 
      Cells.
PG  - 490-498
LID - S2213-6711(17)30273-4 [pii]
LID - 10.1016/j.stemcr.2017.06.010 [doi]
AB  - During liver development, hepatoblasts and liver non-parenchymal cells (NPCs) 
      such as liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells 
      (HSCs) constitute the liver bud where they proliferate and differentiate. 
      Accordingly, we reasoned that liver NPCs would support the maturation of 
      hepatocytes derived from human induced pluripotent stem cells (hiPSCs), which 
      usually exhibit limited functions. We found that the transforming growth factor beta 
      and Rho signaling pathways, respectively, regulated the proliferation and 
      maturation of LSEC and HSC progenitors isolated from mouse fetal livers. Based on 
      these results, we have established culture systems to generate LSECs and HSCs 
      from hiPSCs. These hiPSC-derived NPCs exhibited distinctive phenotypes and 
      promoted self-renewal of hiPSC-derived liver progenitor cells (LPCs) over the 
      long term in the two-dimensional culture system without exogenous cytokines and 
      hepatic maturation of hiPSC-derived LPCs. Thus, a functional human liver model 
      can be constructed in vitro from the LPCs, LSECs, and HSCs derived from hiPSCs.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Koui, Yuta
AU  - Koui Y
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Kido, Taketomo
AU  - Kido T
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic 
      address: kido@iam.u-tokyo.ac.jp.
FAU - Ito, Toshimasa
AU  - Ito T
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Oyama, Hiroki
AU  - Oyama H
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Chen, Shin-Wei
AU  - Chen SW
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Katou, Yuki
AU  - Katou Y
AD  - Laboratory of Genome Structure and Function, Research Center for Epigenetic 
      Disease, Institute of Molecular and Cellular Biosciences, The University of 
      Tokyo, Tokyo 113-0032, Japan.
FAU - Shirahige, Katsuhiko
AU  - Shirahige K
AD  - Laboratory of Genome Structure and Function, Research Center for Epigenetic 
      Disease, Institute of Molecular and Cellular Biosciences, The University of 
      Tokyo, Tokyo 113-0032, Japan.
FAU - Miyajima, Atsushi
AU  - Miyajima A
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic 
      address: miyajima@iam.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170727
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (Activated-Leukocyte Cell Adhesion Molecule)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Activated-Leukocyte Cell Adhesion Molecule/metabolism
MH  - Animals
MH  - Biomarkers
MH  - *Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells/cytology/metabolism
MH  - Fetus
MH  - Gene Expression Profiling
MH  - Hepatic Stellate Cells/cytology/metabolism
MH  - Hepatocytes/cytology/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Liver/*cytology/metabolism
MH  - Liver Regeneration
MH  - Mice
MH  - Phenotype
PMC - PMC5549957
OTO - NOTNLM
OT  - hepatic stellate cells
OT  - liver bud
OT  - liver development
OT  - liver progenitor cells
OT  - liver sinusoidal endothelial cells
OT  - pluripotent stem cells
EDAT- 2017/08/02 06:00
MHDA- 2018/04/24 06:00
PMCR- 2017/07/27
CRDT- 2017/08/01 06:00
PHST- 2017/01/14 00:00 [received]
PHST- 2017/06/22 00:00 [revised]
PHST- 2017/06/23 00:00 [accepted]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2017/08/01 06:00 [entrez]
PHST- 2017/07/27 00:00 [pmc-release]
AID - S2213-6711(17)30273-4 [pii]
AID - 10.1016/j.stemcr.2017.06.010 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2017 Aug 8;9(2):490-498. doi: 10.1016/j.stemcr.2017.06.010. 
      Epub 2017 Jul 27.