PMID- 28757758
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1016-3190 (Print)
IS  - 2223-8956 (Electronic)
VI  - 29
IP  - 1
DP  - 2017 Jan-Mar
TI  - Deduced probable human leukocyte antigen haplotypes associated with human 
      leukocyte antigen DRB1*04:36 identified by case analysis of Taiwanese 
      individuals.
PG  - 12-17
LID - 10.4103/tcmj.tcmj_8_17 [doi]
AB  - OBJECTIVE: Human leukocyte antigen (HLA) DRB1*04:36 is a low-frequency HLA-DRB1 
      allele. The aim here is to report the ethnicity of DRB1*04:36 and its associated 
      HLA haplotypes among Taiwanese individuals. MATERIALS AND METHODS: A 
      sequence-based typing method was employed to confirm this low incidence allele. 
      Polymerase chain reaction was performed to amplify exons 2 and 3 of the HLA-A and 
      HLA-B loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The 
      amplicons were sequenced in both directions using BigDye Terminator Cycle 
      Sequencing Ready Reaction kits and the manufacturer's protocols. One group of 
      unrelated blood donors used in this study consists of randomized individuals with 
      Taiwanese ethnicity who participate in the Tzu Chi Bone Marrow Donor Registry and 
      the other group are randomized unrelated individuals from mainland China. The 
      family members in the family part of the study are volunteer blood donors. 
      RESULTS: In exon 2, the DNA sequence of DRB1*04:36 is identical to DRB1*04:03:01 
      except for a nucleotide segment from residue 286 to residue 308. The nucleotide 
      segment from residue 286 to residue 308, incidentally, is identical to that of 
      DRB1*11:01:01:01. These observations suggest that DRB1*04:36 may have been 
      derived through a gene recombination event involving DRB1*04:03:01 and 
      DRB1*11:01:01:01. Our family study indicated that the HLA haplotype in 
      association with DRB1*04:36 can be deduced to be A*24:02-B*39:01-DRB1*04:36. A 
      randomized population study using Taiwanese suggests that additional DRB1*04:36 
      associated HLA haplotypes seem to exist. CONCLUSION: The information on the 
      ethnicity of the DRB1**04:36 allele, and the deduced probable HLA haplotypes 
      associated with the low incidence DRB1*04:36 allele that we report here, is of 
      value to HLA testing laboratories for reference purposes. In addition, they can 
      be used by stem cell transplantation donor search coordinators to aid the 
      creation of strategy for finding compatible donors who are part of unrelated bone 
      marrow donor registries when a patient carries this uncommon HLA allele.
FAU - Yang, Kuo-Liang
AU  - Yang KL
AD  - Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank and Buddhist Tzu Chi Marrow 
      Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, 
      Hualien, Taiwan.
AD  - Department of Laboratory Medicine, Buddhist Tzu Chi University, Hualien, Taiwan.
FAU - Zheng, Zheng-Zhong
AU  - Zheng ZZ
AD  - China Shanghai Tissuebank Diagnostics, Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Ci Ji Yi Xue Za Zhi
JT  - Ci ji yi xue za zhi = Tzu-chi medical journal
JID - 9514171
PMC - PMC5509187
OTO - NOTNLM
OT  - Anthropology
OT  - Chile
OT  - Libya
OT  - Mainland Chinese
OT  - Taiwanese
COIS- There are no conflicts of interest.
EDAT- 2017/08/02 06:00
MHDA- 2017/08/02 06:01
PMCR- 2017/01/01
CRDT- 2017/08/01 06:00
PHST- 2017/08/01 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2017/08/02 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - TCMJ-29-12 [pii]
AID - 10.4103/tcmj.tcmj_8_17 [doi]
PST - ppublish
SO  - Ci Ji Yi Xue Za Zhi. 2017 Jan-Mar;29(1):12-17. doi: 10.4103/tcmj.tcmj_8_17.