PMID- 28757772
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1016-3190 (Print)
IS  - 2223-8956 (Electronic)
VI  - 29
IP  - 2
DP  - 2017 Apr-Jun
TI  - Using high-resolution human leukocyte antigen typing of 11,423 randomized 
      unrelated individuals to determine allelic varieties, deduce probable human 
      leukocyte antigen haplotypes, and observe linkage disequilibria between human 
      leukocyte antigen-B and-C and human leukocyte antigen-DRB1 and-DQB1 alleles in 
      the Taiwanese Chinese population.
PG  - 84-90
LID - 10.4103/tcmj.tcmj_35_17 [doi]
AB  - OBJECTIVE: We report here the human leukocyte antigen (HLA) allelic variety and 
      haplotype composition in a cohort of the Taiwanese Chinese population and their 
      patterns of linkage disequilibria on HLA-B: HLA-C alleles and HLA-DRB1: HLA-DQB1 
      alleles at a high-resolution level. MATERIALS AND METHODS: Peripheral whole blood 
      from 11,423 Taiwanese Chinese unrelated individuals was collected in acid citrate 
      dextrose. Genomic DNA was extracted using the QIAamp DNA Blood Mini Kit. The DNA 
      material was subjected to HLA genotyping for HLA-A,-B,-C,-DRB1, and-DQB1 loci 
      using a commercial polymerase chain reaction-sequence-based typing (PCR-SBT) kit, 
      the SeCore((R)) A/B/C/DRB1/DQB1 Locus Sequencing kit. High-resolution allelic 
      sequencing was performed as previously described. RESULTS: The number of 
      individual HLA-B alleles detected was greater than the number of alleles 
      recognized in the both the HLA-A and-DRB1 loci. Several novel alleles were 
      discovered as a result of employing the SBT method and the high number of donors 
      tested. In addition, we observed a genetic polymorphic feature of association 
      between HLA-A and-B, HLA-B and-C, and HLA-DRB1 and-DQB1 alleles. Further, the 
      homozygous haplotype frequencies of HLA-A and-B; HLA-A,-C, and-B; HLA-A,-C,-B, 
      and-DRB1; and HLA-A,-C,-B,-DRB1, and-DQB1 in Taiwanese Chinese population are 
      presented. CONCLUSION: As increasing number of HLA alleles are being discovered, 
      periodic HLA profile investigation in a given population is essential to 
      recognize the HLA complexity in that population. Population study can also 
      provide an up-to-date strategic plan for future needs in terms of compatibility 
      measurement for HLA matching between transplant donors and patients.
FAU - Yang, Kuo-Liang
AU  - Yang KL
AD  - Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank and Buddhist Tzu Chi Marrow 
      Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, 
      Hualien, Taiwan.
AD  - Department of Laboratory Medicine, Buddhist Tzu Chi University, Hualien, Taiwan.
FAU - Chen, Hsee-Bin
AU  - Chen HB
AD  - Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank and Buddhist Tzu Chi Marrow 
      Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, 
      Hualien, Taiwan.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Ci Ji Yi Xue Za Zhi
JT  - Ci ji yi xue za zhi = Tzu-chi medical journal
JID - 9514171
PMC - PMC5509198
OTO - NOTNLM
OT  - Alleles
OT  - Haplotypes
OT  - Human leukocyte antigen
OT  - Induced pluripotent stem cells
OT  - Linkage disequilibria
OT  - Taiwanese
COIS- There are no conflicts of interest.
EDAT- 2017/08/02 06:00
MHDA- 2017/08/02 06:01
PMCR- 2017/04/01
CRDT- 2017/08/01 06:00
PHST- 2017/08/01 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2017/08/02 06:01 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - TCMJ-29-84 [pii]
AID - 10.4103/tcmj.tcmj_35_17 [doi]
PST - ppublish
SO  - Ci Ji Yi Xue Za Zhi. 2017 Apr-Jun;29(2):84-90. doi: 10.4103/tcmj.tcmj_35_17.