PMID- 28759334
OWN - NLM
STAT- MEDLINE
DCOM- 20180927
LR  - 20190107
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 31
IP  - 1
DP  - 2018 Jan/Feb
TI  - B Cell Tetramer Development for Veterinary Vaccinology.
PG  - 1-10
LID - 10.1089/vim.2017.0073 [doi]
AB  - Immunological memory is elicited after either vaccination or natural exposure to 
      a pathogen and is essential for protection against re-exposure. Despite its 
      critical importance, the ability to interrogate the veterinary animal memory 
      immune response has long been hindered by a paucity of tools to assess 
      immunological memory. As a result, the evaluation and analysis of protective 
      immune responses that predict immune protection in food and fiber animals and 
      facilitate vaccine development are obstructed. To fill this gap in knowledge in 
      swine, we created a B cell tetramer to porcine reproductive and respiratory 
      syndrome virus (PRRSV) nonstructural protein 7 (nsp7) to efficiently and 
      effectively investigate the memory B cell response, a hallmark of anti-viral 
      immunity. This novel reagent was validated by using a modified capture ELISA, 
      tetramer pulldowns, and flow cytometry, and it was shown to detect rare, 
      antigen-specific B cells that were present at a frequency of about 0.001% of 
      total B lymphocytes in immune animals. The nsp7-B cell tetramer will help to 
      characterize the PRRSV-specific memory B cell response, which is fundamentally 
      important for understanding immunological competence and animal variation in 
      resistance to PRRSV infection. We expect that the method will be widely 
      applicable to the exploration of immunity to veterinary pathogens.
FAU - Rahe, Michael C
AU  - Rahe MC
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota , St. 
      Paul, Minnesota.
FAU - Gustafson, Kevin L
AU  - Gustafson KL
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota , St. 
      Paul, Minnesota.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota , St. 
      Paul, Minnesota.
LA  - eng
GR  - T32 OD010993/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170731
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Complement 3d)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Animals
MH  - B-Lymphocyte Subsets/*immunology/metabolism
MH  - Biotin/chemistry
MH  - Immunologic Memory/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Protein Multimerization
MH  - Receptors, Antigen, B-Cell/immunology/metabolism
MH  - Receptors, Complement 3d/immunology/metabolism
MH  - Recombinant Proteins/chemistry/immunology
MH  - Sensitivity and Specificity
MH  - Spleen/cytology/immunology
MH  - Swine
MH  - Vaccination/*veterinary
MH  - Viral Nonstructural Proteins/chemistry/*immunology
PMC - PMC5770100
OTO - NOTNLM
OT  - B cell
OT  - ELISA
OT  - PRRSV
OT  - memory
OT  - nsp7
OT  - tetramer
COIS- No competing financial interests exist.
EDAT- 2017/08/02 06:00
MHDA- 2018/09/28 06:00
PMCR- 2019/01/01
CRDT- 2017/08/01 06:00
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/09/28 06:00 [medline]
PHST- 2017/08/01 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.1089/vim.2017.0073 [pii]
AID - 10.1089/vim.2017.0073 [doi]
PST - ppublish
SO  - Viral Immunol. 2018 Jan/Feb;31(1):1-10. doi: 10.1089/vim.2017.0073. Epub 2017 Jul 
      31.