PMID- 28759862
OWN - NLM
STAT- MEDLINE
DCOM- 20180529
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 115
DP  - 2017 Dec
TI  - Genetic diversity in envelope genes of contemporary U.S. porcine reproductive and 
      respiratory syndrome virus strains influences viral antigenicity.
PG  - 432-441
LID - S0034-5288(17)30287-4 [pii]
LID - 10.1016/j.rvsc.2017.07.027 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is one of the most 
      economically important diseases in swine caused by porcine reproductive and 
      respiratory syndrome virus (PRRSV). Genome sequences of sixty-six PRRSV strains 
      were obtained using metagenomic sequencing of serum samples collected in the U.S. 
      in 2014 to explore contemporary genetic diversity. Phylogenetic analysis of the 
      genes encoding the envelope proteins identified four to eight distinct lineages 
      with >87% intraclade identity. To explore the effect of the observed genetic 
      diversity on antigenicity, the genome regions encoding either GP2a-GP3-GP4 or 
      GP5-M in strain SD95-21 were replaced with alleles from each of eight distinct 
      PRRSV strains using reverse genetics. The GP2a-GP3-GP4 region from only four of 
      the eight strains yielded viable recombinant virus. When viable, both 
      GP2a-GP3-GP4 and GP5-M variably affected antigenicity. A strain-dependent 
      significant loss in cross reactivity was variably observed by indirect 
      immunofluorescence assays using antisera from pigs vaccinated with commercial 
      modified-live vaccines following replacement of GP2a-GP3-GP4 or GP5-M. 
      Significantly reduced neutralization titers were similarly measured using 
      antisera from naturally PRRSV-exposed pigs. These results illustrate the need to 
      consider genomic regions besides GP5 for PRRSV epidemiology and vaccination.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Chen, Zhenhai
AU  - Chen Z
AD  - College of Veterinary Medicine, Yangzhou University, Yangzhou, JS, China; 
      Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS, USA. Electronic address: zhenhai@yzu.edu.cn.
FAU - Collin, Emily
AU  - Collin E
AD  - Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 
      Manhattan, KS, USA.
FAU - Peddireddi, Lalitha
AU  - Peddireddi L
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS, USA; Kansas State Veterinary Diagnostic Laboratory, Kansas State 
      University, Manhattan, KS, USA.
FAU - Clement, Travis
AU  - Clement T
AD  - Animal Disease Research and Diagnostic Laboratory, South Dakota State University, 
      Brookings, SD, USA.
FAU - Gauger, Phillip
AU  - Gauger P
AD  - Department of Veterinary Diagnostic and Population Animal Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Hause, Ben M
AU  - Hause BM
AD  - Department of Diagnostic Medicine and Pathobiology, Kansas State University, 
      Manhattan, KS, USA; Kansas State Veterinary Diagnostic Laboratory, Kansas State 
      University, Manhattan, KS, USA. Electronic address: bhause@vet.k-state.edu.
LA  - eng
PT  - Journal Article
DEP - 20170726
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/*genetics/*immunology
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Genetic Variation
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Swine
MH  - United States/epidemiology
MH  - Viral Envelope Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - Envelope gene
OT  - Metagenomic sequencing
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Reverse genetics
EDAT- 2017/08/02 06:00
MHDA- 2018/05/31 06:00
CRDT- 2017/08/01 06:00
PHST- 2017/03/12 00:00 [received]
PHST- 2017/06/10 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2017/08/01 06:00 [entrez]
AID - S0034-5288(17)30287-4 [pii]
AID - 10.1016/j.rvsc.2017.07.027 [doi]
PST - ppublish
SO  - Res Vet Sci. 2017 Dec;115:432-441. doi: 10.1016/j.rvsc.2017.07.027. Epub 2017 Jul 
      26.