PMID- 2876066
OWN - NLM
STAT- MEDLINE
DCOM- 19861112
LR  - 20220310
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 6
IP  - 10
DP  - 1986 Oct
TI  - Brain-derived neurotrophic factor supports the survival of cultured rat retinal 
      ganglion cells.
PG  - 3031-8
AB  - Brain-derived neurotrophic factor (BDNF) is a small, basic protein purified from 
      the mammalian brain that has been shown previously to support the survival of 
      cultured spinal sensory neurons (Barde et al., 1982). In current studies, BDNF 
      was tested for its ability to support the survival of cultured CNS cells isolated 
      from the perinatal rat retina. Both immunofluorescent labeling of Thy-1 and prior 
      retrograde labeling with HRP were used as retinal ganglion cell markers in vitro. 
      With embryonic day (E) 17 retinas, it was found that BDNF allowed the survival of 
      a small subpopulation of neurons (about 7% of the cells plated at this age) 
      identified by the immunofluorescent labeling of Thy-1. No detectable effects were 
      seen when either the total number of cells or the number of tetanus 
      toxin-positive neurons was measured. BDNF also had an effect on cultured neurons 
      retrogradely labeled after HRP injections in the superior colliculi of neonatal 
      rats. The BDNF-responsive population was therefore detected only in retinal 
      cultures with specific markers and identified as consisting of retinal ganglion 
      cells. These cells could be enriched about 80-fold by density gradient 
      centrifugation, and purified ganglion cell cultures were shown to be responsive 
      to BDNF. Whereas with E17 retinas, the number of surviving Thy-1 positive neurons 
      could be kept constant for at least 4 d, the survival of postnatal neurons was 
      only transiently increased by BDNF. We conclude that in the retina, BDNF affects 
      only the survival of ganglion cells in vitro by a direct action on these cells. 
      The results are discussed in terms of target-derived neurotrophic support during 
      development.
FAU - Johnson, J E
AU  - Johnson JE
FAU - Barde, Y A
AU  - Barde YA
FAU - Schwab, M
AU  - Schwab M
FAU - Thoenen, H
AU  - Thoenen H
LA  - eng
GR  - 5F32-NSO7332-02X1/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antigens, Surface)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Thy-1 Antigens)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/physiology
MH  - Antigens, Surface/immunology
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Retina/*drug effects/embryology/physiology
MH  - Retinal Ganglion Cells/*drug effects/immunology
MH  - Thy-1 Antigens
PMC - PMC6568792
EDAT- 1986/10/01 00:00
MHDA- 1986/10/01 00:01
PMCR- 1987/04/01
CRDT- 1986/10/01 00:00
PHST- 1986/10/01 00:00 [pubmed]
PHST- 1986/10/01 00:01 [medline]
PHST- 1986/10/01 00:00 [entrez]
PHST- 1987/04/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.06-10-03031.1986 [doi]
PST - ppublish
SO  - J Neurosci. 1986 Oct;6(10):3031-8. doi: 10.1523/JNEUROSCI.06-10-03031.1986.