PMID- 28761040
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20190606
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 23
DP  - 2017 Aug 1
TI  - Microsatellite and Single Nucleotide Polymorphisms in the Insulin-Like Growth 
      Factor 1 Promoter with Insulin Sensitivity and Insulin Secretion.
PG  - 3722-3736
AB  - BACKGROUND To investigate associations of the CA microsatellite and rs35767, 
      rs5742612, and rs2288377 polymorphisms and the single nucleotide polymorphism 
      (SNP) haplotypes with and without the CA microsatellite in the IGF1 promoter with 
      insulin sensitivity and secretion. MATERIAL AND METHODS The CA microsatellite and 
      SNPs were genotyped in 389 type 2 diabetes mellitus (T2DM) patients. A 75 g oral 
      glucose tolerance test (OGTT) was given to all the participants. Associations of 
      the genotypes and haplotypes with insulin sensitivity, insulin secretion, glucose 
      tolerance, and insulin-like growth factor 1 (IGF1) were analyzed by ANCOVA 
      (general linear model) and multiple linear regression, after controlling for 
      gender, age, and BMI. RESULTS The CA microsatellite, rs35767 polymorphisms, and 
      SNP haplotypes with or without CA showed no significant association with 
      metabolic parameters. The C allele of rs5742612 was found to be associated with 
      decreased insulin sensitivity (HOMA-S index, beta=-0.131, P=0.008; fasting insulin 
      level, beta=0.022, P=0.006) and increased insulin secretion (HOMA-B index, beta=0.099, 
      P=0.008; insulin AUC, beta=0.112, P=0.012). The linear regression model also 
      indicated that the A allele of rs2288377 was associated with decreased insulin 
      sensitivity (HOMA-S index, beta=-0.159, P=0.001; fasting insulin, beta=0.143, P=0.001) 
      and increased insulin secretion (HOMA-B index, beta=0.114, P=0.017; insulin AUC, 
      beta=0.042, P=0.002). CONCLUSIONS The CA microsatellite and rs35767 have no 
      genotype-related difference in insulin sensitivity or secretion. The rs5742612 
      and rs2288377 polymorphisms are significantly associated with insulin biology, 
      with the TT genotype exhibiting higher insulin sensitivity and lower insulin 
      secretion compared with carriers of the C allele and A allele, respectively, 
      mostly attributed to the direct functional roles of the two loci.
FAU - Wang, Ruyao
AU  - Wang R
AD  - Graduate School, Tianjin Medical University, Tianjin, China (mainland).
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
FAU - Xu, Dandan
AU  - Xu D
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
FAU - Liu, Rui
AU  - Liu R
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
FAU - Zhao, Lijie
AU  - Zhao L
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
FAU - Hu, Liling
AU  - Hu L
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
FAU - Wu, Ping
AU  - Wu P
AD  - Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, China 
      (mainland).
LA  - eng
PT  - Journal Article
DEP - 20170801
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (IGF1 protein, human)
RN  - 0 (Insulin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Aged
MH  - Female
MH  - Haplotypes/genetics
MH  - Humans
MH  - Insulin/*genetics
MH  - Insulin Resistance/*genetics
MH  - Insulin-Like Growth Factor I/*genetics
MH  - Linear Models
MH  - Male
MH  - Microsatellite Repeats/*genetics
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - *Promoter Regions, Genetic
PMC - PMC5549718
COIS- Conflict of interests The authors have no conflict of interests, and they didn't 
      receive any financial support for this study.
EDAT- 2017/08/02 06:00
MHDA- 2018/04/24 06:00
PMCR- 2017/08/01
CRDT- 2017/08/02 06:00
PHST- 2017/08/02 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 902956 [pii]
AID - 10.12659/msm.902956 [doi]
PST - epublish
SO  - Med Sci Monit. 2017 Aug 1;23:3722-3736. doi: 10.12659/msm.902956.