PMID- 28761985
OWN - NLM
STAT- MEDLINE
DCOM- 20171212
LR  - 20181113
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 471
IP  - 6
DP  - 2017 Dec
TI  - Synovial sarcoma showing loss of a green signal in SS18 fluorescence in situ 
      hybridization: a clinicopathological and molecular study of 12 cases.
PG  - 799-807
LID - 10.1007/s00428-017-2211-2 [doi]
AB  - The phenomenon of losing a green signal in synovial sarcoma (SS) using the SS18 
      break-apart probe by fluorescence in situ hybridization (FISH) has been poorly 
      described. In this study, 12 SS with missing a green signal were identified. This 
      series included 7 males and 5 females, aged 17 to 69 years (median, 38.5 years). 
      The tumors involved the extremities (50%), mediastinum (16.7%), hypopharynx 
      (8.3%), neck (8.3%), thyroid (8.3%), and retroperitoneum (8.3%). The tumors were 
      classified as monophasic SS (58.3%) and poorly differentiated SS (41.7%). An 
      anaplastic SS showing features of pleomorphic sarcoma was observed. 
      Immunostaining for TLE1, BCL2, CD99, epithelial membrane antigen, cytokeratin 
      (AE1/AE3), cytokeratin 7, S-100 protein, and CD34 was consistent with typical SS. 
      In FISH, all the tumors showed the pattern of 1 to 3 fused signal(s) with 1 to 3 
      red signal(s), without corresponding a green signal. The fusion transcripts 
      included SS18-SSX1 (8/10, 80%) and SS18-SSX2 (2/10, 20%) fusions. Median and 
      5-year overall survival were 19.1 months and 43.6%, respectively. In conclusion, 
      we reported a series of SS losing a green signal in the SS18 FISH assay. We 
      propose that this variant FISH pattern should be interpreted as a peculiar 
      unbalanced rearrangement of the SS18 gene and subsequent SS18-SSX fusion test 
      should be recommended. The cases in this study seem to show some unusual 
      clinicopathological features, including unusual locations, higher proportions of 
      poorly differentiated SS, and aggressive clinical course. However, whether this 
      variant FISH pattern is associated with peculiar clinicopathologic features 
      awaits larger series.
FAU - Jiang, Dan
AU  - Jiang D
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Peng, Ran
AU  - Peng R
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Yan, Xiaochu
AU  - Yan X
AD  - Institute of Pathology and Southwest Cancer Center, Southwest Hospital and Key 
      Laboratory of Tumor Immunopathology of the Ministry of Education of China, Third 
      Military Medical University, Chongqing, 400038, China.
FAU - Chen, Min
AU  - Chen M
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Lan, Ting
AU  - Lan T
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Chen, Huijiao
AU  - Chen H
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Zhang, Zhang
AU  - Zhang Z
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Jing, Wenyi
AU  - Jing W
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Jiang, Lili
AU  - Jiang L
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China.
FAU - Ma, Limei
AU  - Ma L
AD  - Department of Pathology, The Third People's Hospital of Zigong City, Zigong, 
      643020, China.
FAU - Zhang, Hongying
AU  - Zhang H
AD  - Department of Pathology, West China Hospital, Sichuan University, Guoxuexiang 37, 
      Chengdu, Sichuan, 610041, China. hy_zhang@scu.edu.cn.
LA  - eng
GR  - 81472510/National Natural Science Foundation of China/
GR  - 81272944/National Natural Science Foundation of China/
GR  - 81401990/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20170731
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SS18 protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Repressor Proteins/*genetics
MH  - Sarcoma, Synovial/*genetics/pathology
MH  - Young Adult
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Loss of a green signal
OT  - Pleomorphism
OT  - SS18
OT  - Synovial sarcoma
EDAT- 2017/08/02 06:00
MHDA- 2017/12/13 06:00
CRDT- 2017/08/02 06:00
PHST- 2017/05/20 00:00 [received]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/07/09 00:00 [revised]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2017/12/13 06:00 [medline]
PHST- 2017/08/02 06:00 [entrez]
AID - 10.1007/s00428-017-2211-2 [pii]
AID - 10.1007/s00428-017-2211-2 [doi]
PST - ppublish
SO  - Virchows Arch. 2017 Dec;471(6):799-807. doi: 10.1007/s00428-017-2211-2. Epub 2017 
      Jul 31.