PMID- 28762863
OWN - NLM
STAT- MEDLINE
DCOM- 20181009
LR  - 20181113
IS  - 2150-5608 (Electronic)
IS  - 2150-5594 (Print)
IS  - 2150-5594 (Linking)
VI  - 8
IP  - 8
DP  - 2017 Nov 17
TI  - Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T 
      cells or stimulating with AIDS-associated pathogens secrete TNF-alpha to reactivate 
      HIV-1 from latency.
PG  - 1732-1743
LID - 10.1080/21505594.2017.1356535 [doi]
AB  - Elucidation of mechanisms underlying the establishment, maintenance of and 
      reactivation from HIV-1 latency is essential for the development of therapeutic 
      strategies aimed at eliminating HIV-1 reservoirs. Microbial translocation, as a 
      consequence of HIV-1-induced deterioration of host immune system, is known to 
      result in a systemic immune activation and transient outbursts of HIV-1 viremia 
      in chronic HIV-1 infection. How these microbes cause the robust HIV-1 
      reactivation remains elusive. Dendritic cells (DCs) have previously been shown to 
      reactivate HIV-1 from latency; however, the precise role of DCs in reactivating 
      HIV-1 from latently infected T-cell remains obscure. In this study, by using 
      HIV-1 latently infected Jurkat T cells, we demonstrated that AIDS-associated 
      pathogens as represented by Mycobacterium bovis (M. bovis) Bacillus 
      Calmette-Guerin (BCG) and bacterial component lipopolysaccharide (LPS) were 
      unable to directly reactivate HIV-1 from Jurkat T cells; instead, they mature DCs 
      to secrete TNF-alpha to accomplish this goal. Moreover, we found that HIV-1 latently 
      infected Jurkat T cells could also mature DCs and enhance their TNF-alpha production 
      during co-culture in a CD40-CD40L-signaling-dependent manner. This in turn led to 
      viral reactivation from Jurkat T cells. Our results reveal how DCs help 
      AIDS-associated pathogens to trigger HIV-1 reactivation from latency.
FAU - Ren, Xiao-Xin
AU  - Ren XX
AD  - a Jiangsu Key Laboratory of Infection and Immunity , Institutes of Biology and 
      Medical Sciences, Soochow University , Suzhou , China.
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
FAU - Ma, Li
AU  - Ma L
AD  - a Jiangsu Key Laboratory of Infection and Immunity , Institutes of Biology and 
      Medical Sciences, Soochow University , Suzhou , China.
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
FAU - Sun, Wei-Wei
AU  - Sun WW
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
AD  - c University of Chinese Academy of Sciences , Beijing , China.
FAU - Kuang, Wen-Dong
AU  - Kuang WD
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
AD  - c University of Chinese Academy of Sciences , Beijing , China.
FAU - Li, Tai-Sheng
AU  - Li TS
AD  - d Department of Infectious Diseases , Peking Union Medical College Hospital, 
      Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , 
      China.
FAU - Jin, Xia
AU  - Jin X
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
FAU - Wang, Jian-Hua
AU  - Wang JH
AD  - b Key Laboratory of Molecular Virology and Immunology , Institut Pasteur of 
      Shanghai, Chinese Academy of Sciences , Shanghai , China.
AD  - c University of Chinese Academy of Sciences , Beijing , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170816
PL  - United States
TA  - Virulence
JT  - Virulence
JID - 101531386
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
CIN - Virulence. 2017 Nov 17;8(8):1494-1497. doi: 10.1080/21505594.2017.1371898. PMID: 
      28895782
MH  - Acquired Immunodeficiency Syndrome/genetics/*metabolism/virology
MH  - CD4-Positive T-Lymphocytes/immunology/virology
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/*immunology
MH  - HIV-1/genetics/*physiology
MH  - Humans
MH  - Jurkat Cells/drug effects/*virology
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism/*pharmacology
MH  - Virus Activation
MH  - *Virus Latency
PMC - PMC5810491
OTO - NOTNLM
OT  - CD40-CD40L signaling
OT  - HIV-1
OT  - TNF-alpha
OT  - dendritic cells
OT  - viral latency
EDAT- 2017/08/02 06:00
MHDA- 2018/10/10 06:00
PMCR- 2018/08/16
CRDT- 2017/08/02 06:00
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/10/10 06:00 [medline]
PHST- 2017/08/02 06:00 [entrez]
PHST- 2018/08/16 00:00 [pmc-release]
AID - 1356535 [pii]
AID - 10.1080/21505594.2017.1356535 [doi]
PST - ppublish
SO  - Virulence. 2017 Nov 17;8(8):1732-1743. doi: 10.1080/21505594.2017.1356535. Epub 
      2017 Aug 16.