PMID- 28763057
OWN - NLM
STAT- MEDLINE
DCOM- 20180503
LR  - 20181113
IS  - 2158-3188 (Electronic)
IS  - 2158-3188 (Linking)
VI  - 7
IP  - 8
DP  - 2017 Aug 1
TI  - BECon: a tool for interpreting DNA methylation findings from blood in the context 
      of brain.
PG  - e1187
LID - 10.1038/tp.2017.171 [doi]
AB  - Tissue differences are one of the largest contributors to variability in the 
      human DNA methylome. Despite the tissue-specific nature of DNA methylation, the 
      inaccessibility of human brain samples necessitates the frequent use of surrogate 
      tissues such as blood, in studies of associations between DNA methylation and 
      brain function and health. Results from studies of surrogate tissues in humans 
      are difficult to interpret in this context, as the connection between blood-brain 
      DNA methylation is tenuous and not well-documented. Here, we aimed to provide a 
      resource to the community to aid interpretation of blood-based DNA methylation 
      results in the context of brain tissue. We used paired samples from 16 
      individuals from three brain regions and whole blood, run on the Illumina 450 K 
      Human Methylation Array to quantify the concordance of DNA methylation between 
      tissues. From these data, we have made available metrics on: the variability of 
      cytosine-phosphate-guanine dinucleotides (CpGs) in our blood and brain samples, 
      the concordance of CpGs between blood and brain, and estimations of how strongly 
      a CpG is affected by cell composition in both blood and brain through the web 
      application BECon (Blood-Brain Epigenetic Concordance; 
      https://redgar598.shinyapps.io/BECon/). We anticipate that BECon will enable 
      biological interpretation of blood-based human DNA methylation results, in the 
      context of brain.
FAU - Edgar, R D
AU  - Edgar RD
AD  - Department of Medical Genetics, University of British Columbia, Vancouver, BC, 
      Canada.
AD  - Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, 
      Vancouver, BC, Canada.
FAU - Jones, M J
AU  - Jones MJ
AD  - Department of Medical Genetics, University of British Columbia, Vancouver, BC, 
      Canada.
AD  - Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, 
      Vancouver, BC, Canada.
FAU - Meaney, M J
AU  - Meaney MJ
AD  - Ludmer Centre for Neuroinformatics and Mental Health, Douglas Mental Health 
      University Institute, McGill University, Montreal, QC, Canada.
AD  - Singapore Institute for Clinical Sciences, Singapore.
AD  - Canadian Institute for Advanced Research, Toronto, ON, Canada.
FAU - Turecki, G
AU  - Turecki G
AD  - Department of Psychiatry, McGill University, Montreal, QC, Canada.
FAU - Kobor, M S
AU  - Kobor MS
AD  - Department of Medical Genetics, University of British Columbia, Vancouver, BC, 
      Canada.
AD  - Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, 
      Vancouver, BC, Canada.
AD  - Canadian Institute for Advanced Research, Toronto, ON, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170801
PL  - United States
TA  - Transl Psychiatry
JT  - Translational psychiatry
JID - 101562664
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Brain/*metabolism
MH  - CpG Islands
MH  - DNA/*blood
MH  - DNA Methylation
MH  - Epigenomics/*methods
MH  - Humans
PMC - PMC5611738
COIS- The authors declare no conflict of interest.
EDAT- 2017/08/02 06:00
MHDA- 2018/05/04 06:00
PMCR- 2017/08/01
CRDT- 2017/08/02 06:00
PHST- 2017/02/24 00:00 [received]
PHST- 2017/06/14 00:00 [revised]
PHST- 2017/06/17 00:00 [accepted]
PHST- 2017/08/02 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2018/05/04 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - tp2017171 [pii]
AID - 10.1038/tp.2017.171 [doi]
PST - epublish
SO  - Transl Psychiatry. 2017 Aug 1;7(8):e1187. doi: 10.1038/tp.2017.171.