PMID- 28763481
OWN - NLM
STAT- MEDLINE
DCOM- 20171010
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Osteoclastic differentiation and resorption is modulated by bioactive metal ions 
      Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements.
PG  - e0182109
LID - 10.1371/journal.pone.0182109 [doi]
LID - e0182109
AB  - Biologically active metal ions in low doses have the potential to accelerate bone 
      defect healing. For successful remodelling the interaction of bone graft 
      materials with both bone-forming osteoblasts and bone resorbing osteoclasts is 
      crucial. In the present study brushite forming calcium phosphate cements (CPC) 
      were doped with Co2+, Cu2+ and Cr3+ and the influence of these materials on 
      osteoclast differentiation and activity was examined. Human osteoclasts were 
      differentiated from human peripheral blood mononuclear cells (PBMC) both on the 
      surface and in indirect contact to the materials on dentin discs. Release of 
      calcium, phosphate and bioactive metal ions was determined using ICP-MS both in 
      the presence and absence of the cells. While Co2+ and Cu2+ showed a burst 
      release, Cr3+ was released steadily at very low concentrations (below 1 muM) and 
      both calcium and phosphate release of the cements was considerably changed in the 
      Cr3+ modified samples. Direct cultivation of PBMC/osteoclasts on Co2+ cements 
      showed lower attached cell number compared to the reference but high activity of 
      osteoclast specific enzymes tartrate resistant acid phosphatase (TRAP), carbonic 
      anhydrase II (CAII) and cathepsin K (CTSK) and significantly increased gene 
      expression of vitronectin receptor. Indirect cultivation with diluted Co2+ cement 
      extracts revealed highest resorbed area compared to all other modifications and 
      the reference. Cu2+ cements had cytotoxic effect on PBMC/osteoclasts during 
      direct cultivation, while indirect cultivation with diluted extracts from Cu2+ 
      cements did not provoke cytotoxic effects but a strictly inhibited resorption. 
      Cr3+ doped cements did not show cytotoxic effects at all. Gene expression and 
      enzyme activity of CTSK was significantly increased in direct culture. Indirect 
      cultivation with Cr3+ doped cements revealed significantly higher resorbed area 
      compared to the reference. In conclusion Cr3+ doped calcium phosphate cements are 
      an innovative cement modification because of their high cytocompatibility and 
      support of active resorption by osteoclasts.
FAU - Bernhardt, Anne
AU  - Bernhardt A
AD  - Centre for Translational Bone, Joint and Soft Tissue Research, University 
      Hospital Carl Gustav Carus and Faculty of Medicine of Technische Universitat 
      Dresden, Dresden, Germany.
FAU - Schamel, Martha
AU  - Schamel M
AD  - Department for Functional Materials in Medicine and Dentistry, University of 
      Wurzburg, Wurzburg, Germany.
FAU - Gbureck, Uwe
AU  - Gbureck U
AD  - Department for Functional Materials in Medicine and Dentistry, University of 
      Wurzburg, Wurzburg, Germany.
FAU - Gelinsky, Michael
AU  - Gelinsky M
AD  - Centre for Translational Bone, Joint and Soft Tissue Research, University 
      Hospital Carl Gustav Carus and Faculty of Medicine of Technische Universitat 
      Dresden, Dresden, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170801
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Ions)
RN  - 0 (Metals)
RN  - 0R0008Q3JB (Chromium)
RN  - 3G0H8C9362 (Cobalt)
RN  - 789U1901C5 (Copper)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Bone Cements/*chemistry
MH  - Bone Resorption
MH  - Calcium Phosphates/chemistry
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Chromium/*chemistry
MH  - Cobalt/*chemistry
MH  - Copper/*chemistry
MH  - Humans
MH  - Inflammation
MH  - Ions/chemistry
MH  - Leukocytes, Mononuclear/cytology
MH  - Metals/chemistry
MH  - Osteoblasts/metabolism
MH  - Osteoclasts/*cytology
MH  - Osteogenesis
MH  - Stem Cells/cytology
PMC - PMC5538673
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/08/02 06:00
MHDA- 2017/10/11 06:00
PMCR- 2017/08/01
CRDT- 2017/08/02 06:00
PHST- 2017/03/26 00:00 [received]
PHST- 2017/07/12 00:00 [accepted]
PHST- 2017/08/02 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - PONE-D-17-11805 [pii]
AID - 10.1371/journal.pone.0182109 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 1;12(8):e0182109. doi: 10.1371/journal.pone.0182109. 
      eCollection 2017.