PMID- 28764292
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2249-782X (Print)
IS  - 0973-709X (Electronic)
IS  - 0973-709X (Linking)
VI  - 11
IP  - 6
DP  - 2017 Jun
TI  - Focal Adhesion Kinase Expression in Ameloblastoma: A Preliminary Observational 
      Study.
PG  - ZC47-ZC50
LID - 10.7860/JCDR/2017/28895.10064 [doi]
AB  - INTRODUCTION: Focal Adhesion Kinase (FAK) is a membrane-associated molecule, 
      which plays a major role in tumourigenesis by promoting cell survival, 
      proliferation, migration, and invasion. Hence, FAK is a well known molecule that 
      has been targeted therapeutically for the treatment of various malignancies. 
      Ameloblastoma (AB) is a locally invasive, destructive and recurrent benign 
      odontogenic lesion. Based on these properties, we have hypothesized that 
      odontogenic epithelium of AB could show increased expression of FAK. AIM: To 
      study the expression of FAK in odontogenic epithelium of AB using 
      immunohistochemistry. MATERIALS AND METHODS: Thirty four paraffin embedded AB and 
      17 Dental Follicle (DF) tissues were retrieved from archives. Immunohistochemical 
      staining was performed using FAK antibody on 5 mum thick formalin fixed paraffin 
      embedded sections. The expression of FAK was studied in ameloblast like cells and 
      stellate reticulum-like cells and was graded as negative, weak and strong. The 
      data was analysed using chi-square test and Fisher's-exact test. RESULTS: In AB, 
      both ameloblast like and stellate reticulum like cells showed positive expression 
      of FAK in the cytoplasm in all the cases (34). The epithelial islands from all 
      the DFs (17) were negative for FAK expression. The difference in the expression 
      of FAK in ameloblast like and stellate reticulum like cells was statistically 
      significant (p<0.0001). Statistically significant difference was observed in the 
      expression of FAK in stellate reticulum like cells of AB and odontogenic 
      epithelial islands of DF (p = 0.0017). All the AB cases showed strong expression 
      of FAK in ameloblast like cells and negative expression was seen in all the cases 
      of DF. CONCLUSION: FAK was strongly expressed in AB as compared to epithelial 
      islands of DF. FAK could be responsible for the neoplastic nature of AB and 
      possibly play a role in tumour invasiveness.
FAU - Patil, Snehal
AU  - Patil S
AD  - Postgraduate Student, Department of Oral Pathology and Microbiology, Dr. D. Y. 
      Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, 
      Pimpri, Pune, Maharashtra, India.
FAU - Sarode, Gargi Sachin
AU  - Sarode GS
AD  - Associate Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. 
      Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, 
      Pimpri, Pune, Maharashtra, India.
FAU - Sarode, Sachin C
AU  - Sarode SC
AD  - Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental 
      College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, 
      Pune, Maharashtra, India.
FAU - Anand, Rahul
AU  - Anand R
AD  - Postgraduate Student, Department of Oral Pathology and Microbiology, Dr. D. Y. 
      Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, 
      Pimpri, Pune, Maharashtra, India.
FAU - Patil, Shankargouda
AU  - Patil S
AD  - Associate Professor, Department of Oral and Maxillofacial Surgery, Division of 
      Oral Pathology, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi 
      Arabia.
LA  - eng
PT  - Journal Article
DEP - 20170601
PL  - India
TA  - J Clin Diagn Res
JT  - Journal of clinical and diagnostic research : JCDR
JID - 101488993
PMC - PMC5535481
OTO - NOTNLM
OT  - Dental follicle
OT  - Immunohistochemistry
OT  - Odontogenic tumour
OT  - Pathogenesis
EDAT- 2017/08/03 06:00
MHDA- 2017/08/03 06:01
PMCR- 2017/08/01
CRDT- 2017/08/03 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/05/05 00:00 [accepted]
PHST- 2017/08/03 06:00 [entrez]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2017/08/03 06:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10.7860/JCDR/2017/28895.10064 [doi]
PST - ppublish
SO  - J Clin Diagn Res. 2017 Jun;11(6):ZC47-ZC50. doi: 10.7860/JCDR/2017/28895.10064. 
      Epub 2017 Jun 1.