PMID- 28764934
OWN - NLM
STAT- MEDLINE
DCOM- 20180518
LR  - 20211204
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1672
DP  - 2017 Oct 1
TI  - Effects of anterior thalamic nuclei deep brain stimulation on neurogenesis in 
      epileptic and healthy rats.
PG  - 65-72
LID - S0006-8993(17)30319-0 [pii]
LID - 10.1016/j.brainres.2017.07.021 [doi]
AB  - BACKGROUND: The efficacy of anterior thalamic nuclei (ANT) deep brain stimulation 
      (DBS) in mitigating epileptic seizures has been established. Though the 
      neuroprotection of ANT-DBS has been illustrated, the seizure mitigating mechanism 
      of ANT-DBS has not been thoroughly elucidated. In particular, the effect of 
      ANT-DBS on neurogenesis has not been reported previously. METHOD: Thirty-two male 
      Sprague Dawley rats were randomly assigned to the following groups: 
      sham-DBS-healthy (HL) (n=8), DBS-HL (n=8), sham-DBS-epilepsy (EP) (n=8) and 
      DBS-EP (n=8). Normal saline and kainic acid were injected, respectively, into the 
      former and later two groups, and seizures were monitored. One month later, rats 
      received electrode implantation. Stimulation was exerted in the DBS group but not 
      in the sham-DBS group. Next, all rats were sacrificed, and the ipsilateral 
      hippocampus was dissected and prepared for quantitative real time PCR (qPCR) and 
      western blot analysis in order to measure neuronal nuclear (NeuN), brain-derived 
      neurotrophic factor (BDNF), doublecortin (DCX) and Ki-67 expressions. RESULTS: A 
      44.4% seizure frequency reduction was obtained after ANT-DBS, and no seizures was 
      observed in healthy rats. NeuN, BDNF, Ki-67 and DCX expression levels were 
      significantly decreased in the epileptic rats compared to healthy rats (P<0.01 or 
      P<0.05). Obvious increases in NeuN, Ki-67 and DCX expressions were observed in 
      epileptic and healthy rats receiving stimulation compared to rats receiving no 
      stimulation (all Ps<0.01). However, BDNF expression was not affected by ANT-DBS 
      (all Ps>0.05). CONCLUSIONS: (1) ANT-DBS reduces neuronal loss during the chronic 
      stage of epilepsy. (2) Neurogenesis is elevated by ANT-DBS in both epileptic and 
      healthy rats, and this elevation may not be regulated via a BDNF pathway.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Chen, Ying-Chuan
AU  - Chen YC
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: chenyingchuancyc@163.com.
FAU - Shi, Lin
AU  - Shi L
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: shilin2015@foxmail.com.
FAU - Zhu, Guan-Yu
AU  - Zhu GY
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: 375449969@qq.com.
FAU - Wang, Xiu
AU  - Wang X
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: 805064590@qq.com.
FAU - Liu, De-Feng
AU  - Liu DF
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: liudefeng0926@foxmail.com.
FAU - Liu, Yu-Ye
AU  - Liu YY
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China. Electronic address: break_go_1217@126.com.
FAU - Jiang, Yin
AU  - Jiang Y
AD  - Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital 
      Medical University, Beijing 100050, China. Electronic address: 
      jiangyin0802@foxmail.com.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital 
      Medical University, Beijing 100050, China. Electronic address: zxin05@126.com.
FAU - Zhang, Jian-Guo
AU  - Zhang JG
AD  - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China; Department of Functional Neurosurgery, Beijing 
      Neurosurgical Institute, Capital Medical University, Beijing 100050, China; 
      Beijing Key Laboratory of Neurostimulation, Beijing 100050, China. Electronic 
      address: zjguo73@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170729
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Doublecortin Protein)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neuropeptides)
SB  - IM
MH  - Animals
MH  - Anterior Thalamic Nuclei/metabolism/*physiology
MH  - Brain-Derived Neurotrophic Factor/analysis
MH  - Deep Brain Stimulation/*methods
MH  - Doublecortin Domain Proteins
MH  - Doublecortin Protein
MH  - Electrodes, Implanted
MH  - Epilepsy/*therapy
MH  - Hippocampus
MH  - Ki-67 Antigen/analysis
MH  - Male
MH  - Microtubule-Associated Proteins/analysis
MH  - Neurogenesis/physiology
MH  - Neuropeptides/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/therapy
OTO - NOTNLM
OT  - Anterior thalamic nuclei
OT  - Deep brain stimulation
OT  - Epilepsy
OT  - Neurogenesis
OT  - Neuronal loss
EDAT- 2017/08/03 06:00
MHDA- 2018/05/19 06:00
CRDT- 2017/08/03 06:00
PHST- 2017/06/13 00:00 [received]
PHST- 2017/07/23 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2018/05/19 06:00 [medline]
PHST- 2017/08/03 06:00 [entrez]
AID - S0006-8993(17)30319-0 [pii]
AID - 10.1016/j.brainres.2017.07.021 [doi]
PST - ppublish
SO  - Brain Res. 2017 Oct 1;1672:65-72. doi: 10.1016/j.brainres.2017.07.021. Epub 2017 
      Jul 29.