PMID- 28765275
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20231112
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 175
IP  - 1
DP  - 2017 Sep
TI  - Proline Accumulation Is Regulated by Transcription Factors Associated with 
      Phosphate Starvation.
PG  - 555-567
LID - 10.1104/pp.17.00791 [doi]
AB  - Pro accumulation in plants is a well-documented physiological response to osmotic 
      stress caused by drought or salinity. In Arabidopsis (Arabidopsis thaliana), the 
      stress and ABA-induced Delta1-PYRROLINE-5-CARBOXYLATE SYNTHETASE1 (P5CS1) gene was 
      previously shown to control Pro biosynthesis in such adverse conditions. To 
      identify regulatory factors that control the transcription of P5CS1, Y1H screens 
      were performed with a genomic fragment of P5CS1, containing 1.2-kB promoter and 
      0.8-kb transcribed regions. The myeloblastosis (MYB)-type transcription factors 
      PHOSPHATE STARVATION RESPONSE1 (PHR1) and PHR1-LIKE1 (PHL1) were identified to 
      bind to P5CS1 regulatory sequences in the first intron, which carries a conserved 
      PHR1-binding site (P1BS) motif. Binding of PHR1 and PHL1 factors to P1BS was 
      confirmed by Y1H, electrophoretic mobility assay and chromatin 
      immunoprecipitation. Phosphate starvation led to gradual increase in Pro content 
      in wild-type Arabidopsis plants as well as transcriptional activation of P5CS1 
      and PRO DEHYDROGENASE2 genes. Induction of P5CS1 transcription and Pro 
      accumulation during phosphate deficiency was considerably reduced by phr1 and 
      phl1 mutations and was impaired in the ABA-deficient aba2-3 and ABA-insensitive 
      abi4-1 mutants. Growth and viability of phr1phl1 double mutant was significantly 
      reduced in phosphate-depleted medium, while growth was only marginally affected 
      in the aba2-3 mutants, suggesting that ABA is implicated in growth retardation in 
      such nutritional stress. Our results reveal a previously unknown link between Pro 
      metabolism and phosphate nutrition and show that Pro biosynthesis is target of 
      cross talk between ABA signaling and regulation of phosphate homeostasis through 
      PHR1- and PHL1-mediated transcriptional activation of the P5CS1 gene.
CI  - (c) 2017 American Society of Plant Biologists. All Rights Reserved.
FAU - Aleksza, David
AU  - Aleksza D
AD  - Institute of Plant Biology, Biological Research Centre, 6726-Szeged, Hungary.
FAU - Horvath, Gabor V
AU  - Horvath GV
AD  - Institute of Plant Biology, Biological Research Centre, 6726-Szeged, Hungary.
FAU - Sandor, Gyorgyi
AU  - Sandor G
AD  - Institute of Plant Biology, Biological Research Centre, 6726-Szeged, Hungary.
FAU - Szabados, Laszlo
AU  - Szabados L
AUID- ORCID: 0000-0002-9163-9875
AD  - Institute of Plant Biology, Biological Research Centre, 6726-Szeged, Hungary 
      szabados.laszlo@brc.mta.hu.
LA  - eng
PT  - Journal Article
DEP - 20170801
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (PHL1 protein, Arabidopsis)
RN  - 0 (PHR1 protein, Arabidopsis)
RN  - 0 (Phosphates)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Pyrroles)
RN  - 0 (Transcription Factors)
RN  - 0 (delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis)
RN  - 2906-39-0 (delta-1-pyrroline-5-carboxylate)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 1.2.1.41 (Glutamate-5-Semialdehyde Dehydrogenase)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
SB  - IM
MH  - Abscisic Acid/metabolism
MH  - Arabidopsis/*genetics/metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Binding Sites
MH  - Glutamate-5-Semialdehyde Dehydrogenase/genetics/*metabolism
MH  - Multienzyme Complexes/genetics/*metabolism
MH  - Mutation
MH  - Phosphates/deficiency
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics/*metabolism
MH  - Plant Growth Regulators/metabolism
MH  - Proline/*metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Pyrroles/metabolism
MH  - *Signal Transduction
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcriptional Activation
PMC - PMC5580772
EDAT- 2017/08/03 06:00
MHDA- 2018/02/27 06:00
PMCR- 2017/08/01
CRDT- 2017/08/03 06:00
PHST- 2017/06/13 00:00 [received]
PHST- 2017/07/11 00:00 [accepted]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
PHST- 2017/08/03 06:00 [entrez]
PHST- 2017/08/01 00:00 [pmc-release]
AID - pp.17.00791 [pii]
AID - PP2017RA00791DR1 [pii]
AID - 10.1104/pp.17.00791 [doi]
PST - ppublish
SO  - Plant Physiol. 2017 Sep;175(1):555-567. doi: 10.1104/pp.17.00791. Epub 2017 Aug 
      1.