PMID- 28765970
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20210109
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 3
DP  - 2017 Sep
TI  - BMP-7 enhances SnoN mRNA expression in renal tubular epithelial cells under 
      high-glucose conditions.
PG  - 3308-3314
LID - 10.3892/mmr.2017.7025 [doi]
AB  - The present study aimed to identify any association between bone morphogenetic 
      protein‑7 (BMP‑7) and the expression of the transcriptional co‑repressor 
      Ski‑related novel protein N (SnoN), in addition to alterations in 
      tubulointerstitial fibrosis, during the development and progression of diabetic 
      nephropathy (DN). Streptozotocin was injected into the tail veins of 20 healthy 
      and specific pathogen‑free male Sprague‑Dawley rats. The rats were sacrificed to 
      detect the appropriate biochemical indicators of renal pathological alterations 
      following 24 weeks. Then, various doses of human recombinant BMP‑7 were added to 
      high glucose‑cultured NRK‑52E cells. Immunohistochemistry, immunofluorescence 
      staining and western blotting were used to determine the expression of SnoN, 
      BMP‑7, Smad ubiquitin regulatory factor (Smurf)2, Arkadia, E‑cadherin, alpha‑smooth 
      muscle actin and Collagen III. Reverse transcription‑quantitative polymerase 
      chain reaction was used to detect SnoN mRNA expression. With the progression of 
      DN, the expression of BMP‑7 in rat renal tissue was downregulated, whereas the 
      expression of Smurf2 and Arkadia increased. Furthermore, the expression of SnoN 
      mRNA increased however the expression of SnoN protein decreased, accompanied by 
      renal tubular epithelial cell mesenchymal transition, extracellular matrix (ECM) 
      deposition and severe renal function disorder. The exogenous recombinant human 
      BMP‑7 alleviated high glucose‑induced phenotypic transformation and ECM synthesis 
      of NRK‑52E in vitro and upregulated SnoN transcription and protein expression, 
      however no effect was observed on the expression of Smurf2 and Arkadia. BMP‑7 may 
      ameliorate DN and renal fibrosis via increasing the expression of SnoN mRNA and 
      protein in renal tubular epithelial cells, rather than directly inhibiting the 
      degradation of SnoN by E3 ubiquitin ligase.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
FAU - Xiao, Ying
AU  - Xiao Y
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
FAU - Li, Shuang
AU  - Li S
AD  - Department of Pathophysiology, Guizhou Medical Hospital, Guiyang, Guizhou 550002, 
      P.R. China.
FAU - Shi, Lei
AU  - Shi L
AD  - Department of Pathology, The Second Affiliated Hospital of Guizhou Medical 
      University, Kaili, Guizhou 556000, P.R. China.
FAU - Liu, Lirong
AU  - Liu L
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
FAU - Zhang, Yingying
AU  - Zhang Y
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
FAU - Shi, Mingjun
AU  - Shi M
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
FAU - Guo, Bing
AU  - Guo B
AD  - Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou 
      550025, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170717
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Bmp7 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Skil_v1 protein, rat)
RN  - 0 (Transcription Factors)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 7/*metabolism
MH  - Cell Line
MH  - Diabetic Nephropathies/metabolism/pathology
MH  - Epithelial Cells/*metabolism/pathology
MH  - Fibrosis
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose/*pharmacology
MH  - Kidney Tubules/*metabolism/pathology
MH  - Male
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcription Factors/*biosynthesis
PMC - PMC5548011
EDAT- 2017/08/03 06:00
MHDA- 2018/04/24 06:00
PMCR- 2017/07/17
CRDT- 2017/08/03 06:00
PHST- 2016/12/22 00:00 [received]
PHST- 2017/07/04 00:00 [accepted]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2017/08/03 06:00 [entrez]
PHST- 2017/07/17 00:00 [pmc-release]
AID - mmr-16-03-3308 [pii]
AID - 10.3892/mmr.2017.7025 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Sep;16(3):3308-3314. doi: 10.3892/mmr.2017.7025. Epub 2017 Jul 
      17.