PMID- 28766061
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20181202
IS  - 1573-675X (Electronic)
IS  - 1360-8185 (Print)
IS  - 1360-8185 (Linking)
VI  - 22
IP  - 10
DP  - 2017 Oct
TI  - Lysophosphatidic acid enhances human umbilical cord mesenchymal stem cell 
      viability without differentiation via LPA receptor mediating manner.
PG  - 1296-1309
LID - 10.1007/s10495-017-1399-6 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential stromal 
      cells which are regarded as the most feasible stem cell group in cell therapy. 
      The maintenance of cell survival without differentiation is important in cell 
      transplantation and stem cell therapy. However, negative factors exist in cell 
      transplantation. Lysophosphatidic acid (LPA) is a non-antigenic small molecule 
      phospholipid which induced several fundamental cellular responses, such as cell 
      proliferation, apoptosis and migration. In this study we aimed to explore the 
      effects of LPA on the survival and differentiation of MSCs and its availability 
      in cell therapy. We found that LPA stimulated hUC-MSC proliferation and protected 
      hUC-MSCs from lipopolysaccharide (LPS) induced apoptosis. We also observed that 
      CD29, CD44, CD73, CD90 and CD105 were expressed, whereas CD34 and CD45 were not 
      expressed in hUC-MSCs, and these makers have no change in LPA containing medium, 
      which indicated that LPA accelerated the survival of hUC-MSCs in an 
      undifferentiating status. We also demonstrated that higher expressed LPAR1 
      involved in LPA stimulated cell survival action. LPA stimulated cell 
      proliferation was associated with LPAR1 mediated G(i/o)-proteins/ERK1/2 pathway. 
      On the other hand, LPA protected hUC-MSCs from LPS-induced apoptosis through 
      suppressing caspase-3 activation by LPAR1 coupled with a G protein, but not 
      G(i/o) or G(q/11) in hUC-MSC. Collectively, this study demonstrated that LPA 
      increased the proliferation and survival of hUC-MSCs without differentiation 
      through LPAR1 mediated manner. Our findings provide that LPA as a anti-apoptotic 
      agent having potential application prospect in cell transplantation and stem cell 
      therapy.
FAU - Li, Narengerile
AU  - Li N
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China.
AD  - Department of Respiratory and Critical Medicine, The Third Affiliated Hospital, 
      Inner Mongolia Medical University, Baotou, 014010, Inner Mongolia, China.
FAU - Yan, Ya-Li
AU  - Yan YL
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China.
FAU - Fu, Sachaofu
AU  - Fu S
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China.
FAU - Li, Rui-Juan
AU  - Li RJ
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China.
FAU - Zhao, Peng-Fei
AU  - Zhao PF
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China.
FAU - Xu, Xi-Yuan
AU  - Xu XY
AD  - Department of Respiratory and Critical Medicine, The Third Affiliated Hospital, 
      Inner Mongolia Medical University, Baotou, 014010, Inner Mongolia, China.
FAU - Yang, Jing-Ping
AU  - Yang JP
AD  - Department of Respiratory and Critical Medicine, The Third Affiliated Hospital, 
      Inner Mongolia Medical University, Baotou, 014010, Inner Mongolia, China. 
      yangron@sina.com.cn.
FAU - Damirin, Alatangaole
AU  - Damirin A
AD  - Department of Biology, College of Life Sciences, Inner Mongolia University, 
      Hohhot, 010021, Inner Mongolia, China. bigaole@imu.edu.cn.
LA  - eng
GR  - No. 81460021/National Natural Science Foundation of China/International
GR  - No. 8166010065/National Natural Science Foundation of China/International
GR  - No.31260210/National Natural Science Foundation of China/International
PT  - Journal Article
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
RN  - 0 (Antigens, Differentiation)
RN  - 0 (GTP-Binding Protein alpha Subunits)
RN  - 0 (Lysophospholipids)
RN  - 0 (Receptors, Lysophosphatidic Acid)
RN  - EC 3.4.22.- (Caspase 3)
RN  - PG6M3969SG (lysophosphatidic acid)
SB  - IM
MH  - Antigens, Differentiation/genetics
MH  - Apoptosis/drug effects/physiology
MH  - Caspase 3/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects/physiology
MH  - Cell Survival/*drug effects
MH  - Cells, Cultured
MH  - GTP-Binding Protein alpha Subunits/antagonists & inhibitors/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lysophospholipids/pharmacology/*physiology
MH  - MAP Kinase Signaling System/physiology
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Receptors, Lysophosphatidic Acid/antagonists & inhibitors/genetics/*metabolism
MH  - Umbilical Cord/*cytology
PMC - PMC5630659
OTO - NOTNLM
OT  - Cell survival
OT  - Cell-surface marker
OT  - Human umbilical cord mesenchymal stem cells
OT  - Lysophosphatidic acid
OT  - Receptor mediated signaling
COIS- CONFLICT OF INTEREST: The authors declare that they have no conflict of interest. 
      INFORMED CONSENT: Informed consent was obtained from all individual participants 
      included in the study.
EDAT- 2017/08/03 06:00
MHDA- 2018/05/23 06:00
PMCR- 2017/08/01
CRDT- 2017/08/03 06:00
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/08/03 06:00 [entrez]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10.1007/s10495-017-1399-6 [pii]
AID - 1399 [pii]
AID - 10.1007/s10495-017-1399-6 [doi]
PST - ppublish
SO  - Apoptosis. 2017 Oct;22(10):1296-1309. doi: 10.1007/s10495-017-1399-6.