PMID- 28767889
OWN - NLM
STAT- MEDLINE
DCOM- 20180330
LR  - 20180330
IS  - 1678-2690 (Electronic)
IS  - 0001-3765 (Linking)
VI  - 89
IP  - 3
DP  - 2017 Jul-Sep
TI  - Activity of Brazilian propolis against Aeromonas hydrophila and its effect on 
      Nile tilapia growth, hematological and non-specific immune response under 
      bacterial infection.
PG  - 1785-1799
LID - 10.1590/0001-3765201720160630 [doi]
AB  - The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was 
      analyzed by determination of minimum inhibitory concentration (MIC). Then, the 
      effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the 
      Nile tilapia, as well as its effects on resistance to A. hydrophila challenge 
      were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the 
      diet of 280 Nile tilapia (50.0 +/- 5.7 g fish-1). Hemato-immune parameters were 
      analyzed before and after the bacterial challenge. Red blood cell, hematocrit, 
      hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin 
      concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were 
      evaluated. The MIC of the EEP was 13% (v/v) with a bactericidal effect after 24 
      hours. Growth performance was significantly lower for those fish fed diets 
      containing 2.5 and 3% of CPP compared to the control diet. Differences in CPP 
      levels affected fish hemoglobin, neutrophils number and NO following the 
      bacterial challenge. For others parameters no significant differences were 
      observed. Our results show that although propolis has bactericidal properties in 
      vitro, the addition of crude propolis powder to Nile tilapia extruded diets does 
      not necessarily lead to an improvement of fish health.
FAU - Orsi, Ricardo O
AU  - Orsi RO
AD  - Departamento de Producao Animal, Faculdade de Medicina Veterinaria e 
      Zootecnia/FMVZ, Grupo NECTAR, Rua Prof. Doutor Walter Mauricio Correa, s/n, Caixa 
      Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 18618-681 
      Botucatu, SP, Brazil.
FAU - Santos, Vivian G Dos
AU  - Santos VGD
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
FAU - Pezzato, Luiz E
AU  - Pezzato LE
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
FAU - Carvalho, Pedro L P F DE
AU  - Carvalho PLPF
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
FAU - Teixeira, Caroline P
AU  - Teixeira CP
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
FAU - Freitas, Jakeline M A
AU  - Freitas JMA
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
FAU - Padovani, Carlos R
AU  - Padovani CR
AD  - Departamento de Bioestatistica, Instituto de Biociencias de Botucatu, R. Prof. 
      Dr. Antonio Celso Wagner Zanin, 250, Distrito de Rubiao Junior, Universidade 
      Estadual Paulista/UNESP, Campus de Botucatu, 18618-689 Botucatu, SP, Brazil.
FAU - Sartori, Maria M P
AU  - Sartori MMP
AD  - Departamento de Agricultura, Faculdade de Ciencias Agronomicas/FCA, Rua Prof. 
      Doutor Walter Mauricio Correa, s/n, Caixa Postal 560, Universidade Estadual 
      Paulista/UNESP, Campus de Botucatu, 18618-681 Botucatu, SP, Brazil.
FAU - Barros, Margarida M
AU  - Barros MM
AD  - Departamento de Melhoramento e Nutricao Animal, Faculdade de Medicina Veterinaria 
      e Zootecnia/FMVZ, Grupo AQUANUTRI, Rua Prof. Doutor Walter Mauricio Correa, s/n, 
      Caixa Postal 560, Universidade Estadual Paulista/UNESP, Campus de Botucatu, 
      18618-681 Botucatu, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170731
PL  - Brazil
TA  - An Acad Bras Cienc
JT  - Anais da Academia Brasileira de Ciencias
JID - 7503280
RN  - 9009-62-5 (Propolis)
SB  - IM
MH  - Aeromonas hydrophila/*drug effects
MH  - Animals
MH  - Cichlids/blood/*microbiology
MH  - Dietary Supplements
MH  - Fish Diseases/*diet therapy/microbiology
MH  - Gram-Negative Bacterial Infections/diet therapy/microbiology/*veterinary
MH  - Immunity, Innate
MH  - Microbial Sensitivity Tests
MH  - Propolis/*administration & dosage/therapeutic use
EDAT- 2017/08/03 06:00
MHDA- 2018/03/31 06:00
CRDT- 2017/08/03 06:00
PHST- 2017/01/27 00:00 [received]
PHST- 2017/03/21 00:00 [accepted]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2018/03/31 06:00 [medline]
PHST- 2017/08/03 06:00 [entrez]
AID - S0001-37652017005016103 [pii]
AID - 10.1590/0001-3765201720160630 [doi]
PST - ppublish
SO  - An Acad Bras Cienc. 2017 Jul-Sep;89(3):1785-1799. doi: 
      10.1590/0001-3765201720160630. Epub 2017 Jul 31.