PMID- 28768083
OWN - NLM
STAT- MEDLINE
DCOM- 20171207
LR  - 20191113
IS  - 1066-2936 (Print)
IS  - 1066-2936 (Linking)
VI  - 44
IP  - 1
DP  - 2017 Jan-Feb
TI  - Oridonin protects the lung against hyperoxia-induced injury in a mouse model.
PG  - 33-38
AB  - Hyperoxic acute lung injury (HALI) is caused by prolonged exposure to high oxygen 
      partial pressure. This study was undertaken to investigate the protective effects 
      of oridonin on HALI in a mouse model. Mice were randomly divided into three 
      groups: the control group, HALI group and oridonin (ORI) group. HALI was induced 
      by exposing mice to pure oxygen at 2.5 atmospheres absolute (ATA) for six hours 
      in the HALI and ORI groups. In the ORI group, mice were intraperitoneally 
      injected with ORI at 10 mg/kg twice daily after hyperoxic exposure. Animals were 
      sacrificed 24 hours after the hyperoxia exposure, followed by bronchoalveolar 
      lavage fluid (BALF). Lungs were then collected. Each lung was processed for HE 
      staining and detection of wet-to-dry weight ratio. The lactate dehydrogenase 
      (LDH) activity and protein content of BALF were determined, and the contents of 
      malonaldehyde (MDA), glutathione (GSH), tumor necrosis factor alpha (TNF-?) and 
      interleukin-10 (IL-10) in the lung were measured. Our results showed prolonged 
      exposure to hyperoxia significantly damaged the lung, caused lung edema, 
      increased MDA and TNF-?, and reduced GSH and IL-10 in the lung. However, 
      post-exposure treatment with oridonin was able to improve lung pathology, 
      attenuate lung edema, reduce MDA and TNF-?, and increase GSH and IL-10 in the 
      lung. These findings suggest that oridonin can protect the lung against 
      hyperoxia-induced injury in mice.
CI  - Copyright(c) Undersea and Hyperbaric Medical Society.
FAU - Liu, Ying
AU  - Liu Y
AD  - Department of Pathology, Yantaishan Hospital, No 91, Jiefang Road, Zhifu 
      District, Yantai City, Shandong, 264001, P. R. China.
FAU - Zhang, Pei-Xi
AU  - Zhang PX
AD  - Department of Cardiothoracic Surgery, the First People's Hospital of Jining City, 
      No 6, Jiankang Road, Jining City, Shandong, 272011, P. R. China.
FAU - Han, Cui-Hang
AU  - Han CH
AD  - Department of Pathology, the First People's Hospital of Jining City, No 6, 
      Jiankang Road, Jining City, Shandong, 272011, P. R. China.
FAU - Wei, Dunbiao
AU  - Wei D
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
FAU - Qiao, Tongju
AU  - Qiao T
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
FAU - Peng, Bo
AU  - Peng B
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
FAU - Liu, Kan
AU  - Liu K
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
FAU - Zheng, Juan
AU  - Zheng J
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
FAU - Liu, Wenwu
AU  - Liu W
AD  - Department Diving and Hyperbaric Medicine, Secondary Military Medical University, 
      No 800, Xiangyin Road, Yangpu District, Shanghai, 200433, P. R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Undersea Hyperb Med
JT  - Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical 
      Society, Inc
JID - 9312954
RN  - 0 (Diterpenes, Kaurane)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Protective Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0APJ98UCLQ (oridonin)
RN  - 130068-27-8 (Interleukin-10)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - GAN16C9B8O (Glutathione)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acute Lung Injury/*drug therapy/etiology
MH  - Animals
MH  - Atmospheric Pressure
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Disease Models, Animal
MH  - Diterpenes, Kaurane/*therapeutic use
MH  - Glutathione/analysis
MH  - Interleukin-10/analysis
MH  - L-Lactate Dehydrogenase/analysis
MH  - Lung/chemistry
MH  - Malondialdehyde/analysis
MH  - Mice
MH  - Oxygen/*adverse effects
MH  - Partial Pressure
MH  - Protective Agents/*therapeutic use
MH  - Pulmonary Edema/drug therapy/etiology
MH  - Random Allocation
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - hyperbaric oxygenation
OT  - hyperoxic lung injury
OT  - oridonin
COIS- The authors of this paper declare no conflicts of interest exist with this 
      submission.
EDAT- 2017/08/03 06:00
MHDA- 2017/12/08 06:00
CRDT- 2017/08/03 06:00
PHST- 2017/08/03 06:00 [entrez]
PHST- 2017/08/03 06:00 [pubmed]
PHST- 2017/12/08 06:00 [medline]
AID - 10.22462/1.2.2017.6 [doi]
PST - ppublish
SO  - Undersea Hyperb Med. 2017 Jan-Feb;44(1):33-38. doi: 10.22462/1.2.2017.6.