PMID- 28769116
OWN - NLM
STAT- MEDLINE
DCOM- 20190218
LR  - 20240104
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Aug 2
TI  - The Reverse Transcriptases Associated with CRISPR-Cas Systems.
PG  - 7089
LID - 10.1038/s41598-017-07828-y [doi]
LID - 7089
AB  - CRISPR (clustered regularly interspaced short palindromic repeats) and associated 
      proteins (Cas) act as adaptive immune systems in bacteria and archaea. Some 
      CRISPR-Cas systems have been found to be associated with putative reverse 
      transcriptases (RT), and an RT-Cas1 fusion associated with a type III-B system 
      has been shown to acquire RNA spacers in vivo. Nevertheless, the origin and 
      evolutionary relationships of these RTs and associated CRISPR-Cas systems remain 
      largely unknown. We performed a comprehensive phylogenetic analysis of these RTs 
      and associated Cas1 proteins, and classified their CRISPR-Cas modules. These 
      systems were found predominantly in bacteria, and their presence in archaea may 
      be due to a horizontal gene transfer event. These RTs cluster into 12 major 
      clades essentially restricted to particular phyla, suggesting host-dependent 
      functioning. The RTs and associated Cas1 proteins may have largely coevolved. 
      They are, therefore, subject to the same selection pressures, which may have led 
      to coadaptation within particular protein complexes. Furthermore, our results 
      indicate that the association of an RT with a CRISPR-Cas system has occurred on 
      multiple occasions during evolution.
FAU - Toro, Nicolas
AU  - Toro N
AD  - Structure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecologia 
      Genetica de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, 
      Estacion Experimental del Zaidin, Consejo Superior de Investigaciones 
      Cientificas, C/Profesor Albareda 1, 18008, Granada, Spain. 
      nicolas.toro@eez.csic.es.
FAU - Martinez-Abarca, Francisco
AU  - Martinez-Abarca F
AD  - Structure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecologia 
      Genetica de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, 
      Estacion Experimental del Zaidin, Consejo Superior de Investigaciones 
      Cientificas, C/Profesor Albareda 1, 18008, Granada, Spain.
FAU - Gonzalez-Delgado, Alejandro
AU  - Gonzalez-Delgado A
AD  - Structure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecologia 
      Genetica de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, 
      Estacion Experimental del Zaidin, Consejo Superior de Investigaciones 
      Cientificas, C/Profesor Albareda 1, 18008, Granada, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170802
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Archaea/genetics
MH  - Bacteria/genetics
MH  - *CRISPR-Cas Systems
MH  - Genetic Loci
MH  - Genome
MH  - Phylogeny
MH  - Protein Domains/genetics
MH  - RNA-Directed DNA Polymerase/chemistry/*genetics
PMC - PMC5541045
COIS- The authors declare that they have no competing interests.
EDAT- 2017/08/05 06:00
MHDA- 2019/02/20 06:00
PMCR- 2017/08/02
CRDT- 2017/08/04 06:00
PHST- 2017/05/09 00:00 [received]
PHST- 2017/07/03 00:00 [accepted]
PHST- 2017/08/04 06:00 [entrez]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2019/02/20 06:00 [medline]
PHST- 2017/08/02 00:00 [pmc-release]
AID - 10.1038/s41598-017-07828-y [pii]
AID - 7828 [pii]
AID - 10.1038/s41598-017-07828-y [doi]
PST - epublish
SO  - Sci Rep. 2017 Aug 2;7(1):7089. doi: 10.1038/s41598-017-07828-y.