PMID- 28770578
OWN - NLM
STAT- MEDLINE
DCOM- 20180614
LR  - 20180614
IS  - 1365-3164 (Electronic)
IS  - 0959-4493 (Linking)
VI  - 28
IP  - 6
DP  - 2017 Dec
TI  - Detection of IgE-reactive proteins in hydrolysed dog foods.
PG  - 589-e143
LID - 10.1111/vde.12473 [doi]
AB  - BACKGROUND: Commercial hydrolysed diets are used for the diagnosis of food 
      allergy in dogs. The cleaved parent proteins are presumed to be too small to 
      elicit an allergic response by reacting with allergen-specific immunoglobin E 
      (IgE). OBJECTIVES: To evaluate three commercial hydrolysed dog diets for 
      proteins. ANIMALS: Sera were collected from dogs with suspected food allergy. 
      METHODS: Two batches of each hydrolysed diet were examined by electrophoresis and 
      visualized by Coomassie blue, silver nitrate staining and IgE immunoblotting. 
      RESULTS: From two to five proteins, ranging from 21 to 67 kDa, were detected in 
      all three diets evaluated. Circulating IgE antibodies targeting these proteins 
      were detected by immunoblotting of dog sera. Six different carbohydrate proteins 
      were identified by mass spectrometry; maize/potato granule-bound starch 
      synthase-1, soybean glycinin, soybean beta-conglycinin alpha chain, potato aspartic 
      protease inhibitor, rice glutelin type B1 and soybean sucrose-binding protein. 
      Four of these proteins have been described as allergens in humans. CONCLUSIONS: 
      Some commercial hydrolysed diets contain carbohydrate proteins. Some dogs have 
      circulating IgE antibodies targeting these proteins. The clinical significance of 
      these findings is unknown.
CI  - (c) 2017 ESVD and ACVD.
FAU - Roitel, Olivier
AU  - Roitel O
AUID- ORCID: 0000-0001-7688-1500
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Bonnard, Lionel
AU  - Bonnard L
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Stella, Alexandre
AU  - Stella A
AD  - Infrastructure Proteomique de Toulouse, Institut de Pharmacologie et de Biologie 
      Structurale, 205 route de Narbonne BP 64187, 31077, Toulouse, France.
FAU - Schiltz, Odile
AU  - Schiltz O
AD  - Infrastructure Proteomique de Toulouse, Institut de Pharmacologie et de Biologie 
      Structurale, 205 route de Narbonne BP 64187, 31077, Toulouse, France.
FAU - Maurice, Delphine
AU  - Maurice D
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Douchin, Gael
AU  - Douchin G
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Jacquenet, Sandrine
AU  - Jacquenet S
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Favrot, Claude
AU  - Favrot C
AD  - Clinic for Small Animal Internal Medicine, University of Zurich, 
      Winterthurerstrasse 204, Zurich, CH-8057, Switzerland.
FAU - Bihain, Bernard E
AU  - Bihain BE
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
FAU - Couturier, Nicolas
AU  - Couturier N
AD  - Genclis SA, 15 rue du Bois de la Champelle, Vandoeuvre-les-Nancy, 54500, France.
LA  - eng
PT  - Journal Article
DEP - 20170802
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
RN  - 0 (Allergens)
RN  - 0 (Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - *Animal Feed/adverse effects/analysis
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Dog Diseases/*immunology
MH  - *Dogs/immunology
MH  - Electrophoresis, Polyacrylamide Gel/veterinary
MH  - Food Hypersensitivity/immunology/*veterinary
MH  - Immunoglobulin E/*immunology
MH  - Mass Spectrometry/veterinary
MH  - Proteins/analysis/immunology
EDAT- 2017/08/05 06:00
MHDA- 2018/06/15 06:00
CRDT- 2017/08/04 06:00
PHST- 2017/06/11 00:00 [accepted]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2018/06/15 06:00 [medline]
PHST- 2017/08/04 06:00 [entrez]
AID - 10.1111/vde.12473 [doi]
PST - ppublish
SO  - Vet Dermatol. 2017 Dec;28(6):589-e143. doi: 10.1111/vde.12473. Epub 2017 Aug 2.