PMID- 28771523
OWN - NLM
STAT- MEDLINE
DCOM- 20171006
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Differential expression of pathogenic genes of Entamoeba histolytica vs E. dispar 
      in a model of infection using human liver tissue explants.
PG  - e0181962
LID - 10.1371/journal.pone.0181962 [doi]
LID - e0181962
AB  - We sought to establish an ex vivo model for examining the interaction of E. 
      histolytica with human tissue, using precision-cut liver slices (PCLS) from 
      donated organs. E. histolytica- or E. dispar-infected PCLS were analyzed at 
      different post-infection times (0, 1, 3, 24 and 48 h) to evaluate the relation 
      between tissue damage and the expression of genes associated with three factors: 
      a) parasite survival (peroxiredoxin, superoxide dismutase and 70 kDa heat shock 
      protein), b) parasite virulence (EhGal/GalNAc lectin, amoebapore, cysteine 
      proteases and calreticulin), and c) the host inflammatory response (various 
      cytokines). Unlike E. dispar (non-pathogenic), E. histolytica produced some 
      damage to the structure of hepatic parenchyma. Overall, greater expression of 
      virulence genes existed in E. histolytica-infected versus E. dispar-infected 
      tissue. Accordingly, there was an increased expression of EhGal/GalNAc lectin, 
      Ehap-a and Ehcp-5, Ehcp-2, ehcp-1 genes with E. histolytica, and a decreased or 
      lack of expression of Ehcp-2, and Ehap-a genes with E. dispar. E. 
      histolytica-infected tissue also exhibited an elevated expression of genes linked 
      to survival, principally peroxiredoxin, superoxide dismutase and Ehhsp-70. 
      Moreover, E. histolytica-infected tissue showed an overexpression of some genes 
      encoding for pro-inflammatory interleukins (ILs), such as il-8, ifn-gamma and tnf-alpha. 
      Contrarily, E. dispar-infected tissue displayed higher levels of il-10, the gene 
      for the corresponding anti-inflammatory cytokine. Additionally, other genes were 
      investigated that are important in the host-parasite relationship, including 
      those encoding for the 20 kDa heat shock protein (HSP-20), the AIG-1 protein, and 
      immune dominant variable surface antigen, as well as for proteins apparently 
      involved in mechanisms for the protection of the trophozoites in different 
      environments (e.g., thioredoxin-reductase, oxido-reductase, and 9 hypothetical 
      proteins). Some of the hypothetical proteins evidenced interesting overexpression 
      rates, however we should wait to their characterization. This finding suggest 
      that the present model could be advantageous for exploring the complex 
      interaction between trophozoites and hepatocytes during the development of ALA, 
      particularly in the initial stages of infection.
FAU - Ximenez, Cecilia
AU  - Ximenez C
AUID- ORCID: 0000-0003-4777-7047
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Gonzalez, Enrique
AU  - Gonzalez E
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Nieves, Miriam
AU  - Nieves M
AUID- ORCID: 0000-0001-6776-2534
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Magana, Ulises
AU  - Magana U
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Moran, Patricia
AU  - Moran P
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Gudino-Zayas, Marco
AU  - Gudino-Zayas M
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Partida, Oswaldo
AU  - Partida O
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Hernandez, Eric
AU  - Hernandez E
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Rojas-Velazquez, Liliana
AU  - Rojas-Velazquez L
AD  - Laboratory of Immunology, Unit of Experimental Medicine, Faculty of Medicine, 
      UNAM, Mexico City, Mexico.
FAU - Garcia de Leon, Ma Carmen
AU  - Garcia de Leon MC
AUID- ORCID: 0000-0002-7278-6183
AD  - Unit of Scientific Vinculation, Faculty of Medicine, UNAM/INMEGEN, Mexico City, 
      Mexico.
FAU - Maldonado, Hector
AU  - Maldonado H
AD  - Sub direction of Pathology, National Institute of Cancerology, Mexico City, 
      Mexico.
LA  - eng
PT  - Journal Article
DEP - 20170803
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cytokines)
RN  - 0 (Protozoan Proteins)
SB  - IM
EIN - PLoS One. 2019 Jan 10;14(1):e0210895. PMID: 30629702
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cytokines/metabolism
MH  - Entamoeba/*genetics/pathogenicity
MH  - Entamoeba histolytica/*genetics/pathogenicity
MH  - Entamoebiasis/complications/*parasitology
MH  - Female
MH  - Host-Parasite Interactions
MH  - Humans
MH  - Liver Abscess, Amebic/*etiology/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Organ Culture Techniques
MH  - Prevalence
MH  - Protozoan Proteins/*genetics
MH  - Virulence
PMC - PMC5542602
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/08/05 06:00
MHDA- 2017/10/07 06:00
PMCR- 2017/08/03
CRDT- 2017/08/04 06:00
PHST- 2016/12/19 00:00 [received]
PHST- 2017/07/10 00:00 [accepted]
PHST- 2017/08/04 06:00 [entrez]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2017/10/07 06:00 [medline]
PHST- 2017/08/03 00:00 [pmc-release]
AID - PONE-D-16-50026 [pii]
AID - 10.1371/journal.pone.0181962 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 3;12(8):e0181962. doi: 10.1371/journal.pone.0181962. 
      eCollection 2017.