PMID- 28773087
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 10
IP  - 7
DP  - 2017 Jun 30
TI  - Improved Gene Transfer with Functionalized Hollow Mesoporous Silica Nanoparticles 
      of Reduced Cytotoxicity.
LID - 10.3390/ma10070731 [doi]
LID - 731
AB  - Gene therapy is a promising strategy for treatment of genetically caused 
      diseases. Successful gene delivery requires an efficient carrier to transfer the 
      desired gene into host cells. Recently, mesoporous silica nanoparticles (MSNs) 
      functionalized with 25 kD polyethyleneimine (PEI) were extensively used as gene 
      delivery carriers. However, 25 kD PEI could significantly reduce the safety of 
      the modified MSNs although it is efficient for intracellular delivery of nucleic 
      acids. In addition, limited drug loading remains a challenge for conventional 
      MSNs drug carriers. Hollow mesoporous silica nanoparticles (HMSNs) with high pore 
      volume, tunable pore size, and excellent biocompatibility are attractive 
      alternatives. To make them more efficient, a less toxic 1.8 kD PEI polymer was 
      used to functionalize the HMSNs which have large pore size (~10 nm) and form 
      PEI-HMSNs. Scanning and transmission electron microscopic images showed that 
      HMSNs were spherical in shape and approximately 270 nm in diameter with uniform 
      hollow nanostructures. The maximum loading capacity of green fluorescent protein 
      labeled DNA (GFP-DNA) in PEI-HMSNs was found to be 37.98 mg/g. The loading 
      capacity of PEI-HMSNs was nearly three-fold higher than those of PEI modified 
      solid nanoparticles, indicating that both hollow and large pores contributed to 
      the increase in DNA adsorption. The transfection of GFP-DNA plasmid loaded in 
      PEI-HMSNs was increased two-fold in comparison to that of 25 kD PEI. MTT assays 
      in Lovo cells showed that the cell viability was more than 85% when the 
      concentration of PEI-HMSNs was 120 microg/mL, whereas the cell viability was less 
      than 20% when the 25 kD PEI was used at the same concentration. These results 
      indicated that PEI-HMSNs could be used as a delivery system for nucleic acids due 
      to good biocompatibility, high gene loading capacity, and enhanced gene transfer 
      efficiency.
FAU - Zhan, Zhengwen
AU  - Zhan Z
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. zhanzhw@mail2.sysu.edu.cn.
FAU - Zhang, Xiaoxu
AU  - Zhang X
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. zhangxiaoxu@yeah.net.
FAU - Huang, Jiayuan
AU  - Huang J
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. jiayuan.huang@monash.edu.
FAU - Huang, Ying
AU  - Huang Y
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. huangy2007@163.com.
FAU - Huang, Zhengwei
AU  - Huang Z
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. hzhengw3@mail2.sysu.edu.cn.
FAU - Pan, Xin
AU  - Pan X
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. mercurypan@foxmail.com.
AD  - Zhongshan WanYuan New Drug R&D Co., Ltd., Zhongshan 528451, China. 
      mercurypan@foxmail.com.
FAU - Quan, Guilan
AU  - Quan G
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. xiaoplanet@163.com.
FAU - Liu, Hu
AU  - Liu H
AD  - School of Pharmacy, Memorial University of Newfoundland, Newfoundland and 
      Labrador, St. John's, NL A1B 3V6, Canada. chuanbin_wu@126.com.
FAU - Wang, Lili
AU  - Wang L
AD  - School of Pharmacy, Memorial University of Newfoundland, Newfoundland and 
      Labrador, St. John's, NL A1B 3V6, Canada. hliu@mun.ca.
FAU - Wu, And Chuanbin
AU  - Wu AC
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, 
      China. lwang@mun.ca.
LA  - eng
PT  - Journal Article
DEP - 20170630
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC5551774
OTO - NOTNLM
OT  - PEI
OT  - cytotoxicity
OT  - gene transfer
OT  - hollow mesoporous silica nanoparticles
COIS- We declare that we have no financial and personal relationships with other people 
      or organizations that can inappropriately influence our works, there is no 
      professional or other personal interest of any nature or kind in any product, 
      service, and/or company that could be construed as influencing the position 
      presented in, or the review of, the manuscript entitled.
EDAT- 2017/08/05 06:00
MHDA- 2017/08/05 06:01
PMCR- 2017/06/30
CRDT- 2017/08/05 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/06/01 00:00 [revised]
PHST- 2017/06/02 00:00 [accepted]
PHST- 2017/08/05 06:00 [entrez]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2017/08/05 06:01 [medline]
PHST- 2017/06/30 00:00 [pmc-release]
AID - ma10070731 [pii]
AID - materials-10-00731 [pii]
AID - 10.3390/ma10070731 [doi]
PST - epublish
SO  - Materials (Basel). 2017 Jun 30;10(7):731. doi: 10.3390/ma10070731.