PMID- 28774209
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20180814
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 28
IP  - 2
DP  - 2018 Feb
TI  - Analysis of cytotoxic effects of nickel on human blood lymphocytes.
PG  - 79-86
LID - 10.1080/15376516.2017.1364314 [doi]
AB  - Nickel compounds possess many applications in different industrial processes. 
      Human beings are exposed to nickel commonly through occupational exposure and 
      food. Although a few studies so far have investigated the effects of nickel 
      compounds on human lymphocytes, the complete mechanism of cytotoxicity of this 
      metal on human lymphocytes is yet to be determined. The intention of this paper 
      was to determine the cytotoxicity mechanism of water soluble NiCl(2) toward human 
      lymphocytes using the accelerated cytotoxicity mechanisms screening (ACMS) 
      technique. Human lymphocytes were isolated from the blood of healthy subjects 
      based on Ficoll-Paque PLUS standard method. For the assessment of cell viability, 
      lymphocytes were incubated with 0.05-1 mM NiCl(2) for 12 h. Determination of 
      mechanistic parameters was performed 2, 4 and 6 h after treatment of cells with (1/2) 
      EC50(12h), EC50(12h) and 2EC50(12h) of NiCl(2). Our results demonstrate that 
      cytotoxicity of NiCl(2) on human lymphocytes is associated with increased ROS 
      formation, mitochondrial membrane potential collapse, glutathione depletion, 
      lysosomal membrane damage, cellular proteolysis and activation of caspase-3 
      before cytotoxicity ensued.
FAU - Zarei, Mohammad Hadi
AU  - Zarei MH
AD  - a Department of Pharmacology and Toxicology, Faculty of Pharmacy , Shahid 
      Beheshti University of Medical Sciences , Tehran , Iran.
FAU - Hosseini Shirazi, Seyed Farshad
AU  - Hosseini Shirazi SF
AD  - a Department of Pharmacology and Toxicology, Faculty of Pharmacy , Shahid 
      Beheshti University of Medical Sciences , Tehran , Iran.
FAU - Aghvami, Marjan
AU  - Aghvami M
AD  - a Department of Pharmacology and Toxicology, Faculty of Pharmacy , Shahid 
      Beheshti University of Medical Sciences , Tehran , Iran.
FAU - Salimi, Ahmad
AU  - Salimi A
AD  - b Department of Pharmacology and Toxicology, School of Pharmacy , Ardabil 
      University of Medical Science , Ardabil , Iran.
FAU - Pourahmad, Jalal
AU  - Pourahmad J
AD  - a Department of Pharmacology and Toxicology, Faculty of Pharmacy , Shahid 
      Beheshti University of Medical Sciences , Tehran , Iran.
LA  - eng
PT  - Journal Article
DEP - 20171122
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Reactive Oxygen Species)
RN  - 696BNE976J (nickel chloride)
RN  - 7OV03QG267 (Nickel)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Caspase 3/drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Enzyme Activation/drug effects
MH  - Glutathione/metabolism
MH  - Healthy Volunteers
MH  - Humans
MH  - In Vitro Techniques
MH  - Lethal Dose 50
MH  - Lipid Peroxidation/drug effects
MH  - Lymphocytes/*drug effects/metabolism
MH  - Lysosomes/drug effects/metabolism
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Nickel/*toxicity
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Human lymphocytes
OT  - cellular proteolysis
OT  - cytotoxicity
OT  - nickel
EDAT- 2017/08/05 06:00
MHDA- 2018/08/15 06:00
CRDT- 2017/08/05 06:00
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2017/08/05 06:00 [entrez]
AID - 10.1080/15376516.2017.1364314 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2018 Feb;28(2):79-86. doi: 10.1080/15376516.2017.1364314. 
      Epub 2017 Nov 22.