PMID- 28774569
OWN - NLM
STAT- MEDLINE
DCOM- 20180528
LR  - 20181202
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 657
DP  - 2017 Sep 14
TI  - The condition medium of mesenchymal stem cells promotes proliferation, adhesion 
      and neuronal differentiation of retinal progenitor cells.
PG  - 62-68
LID - S0304-3940(17)30626-2 [pii]
LID - 10.1016/j.neulet.2017.07.053 [doi]
AB  - Retinal progenitor cell is a promising candidate in the treatment of retinal 
      pigmentosa diseases. The limiting factors of stem cell transplantation are the 
      proliferation and differentiation capacities of hRPCs, which may be governed by 
      culture conditions. Previous studies have proved that the secretome of human 
      Umbilical Cord Mesenchymal stem cells (hUCMSCs) and human Adipose derived stem 
      cells (hADSCs), including more active cytokines and neurotrophic factors, have 
      the paracrine potential of enhancing proliferation and differentiation in several 
      cell types. The aim of this study was to investigate whether hRPCs could 
      effectively proliferate, adhere and differentiate towards specific retinal cell 
      types by treating with the condition medium (CM) of hUCMSCs (hUCMSCCM) or hADSCs 
      (hADSCCM). Here, we show that hUCMSCCM or hADSCCM enhances the proliferation rate 
      of the S and G2 phase cells, with an upregulation of Ki67 expression. Moreover, 
      the upregulation expression of NF, Recoverin and Rhodopsin indicates that 
      specialized retinal cells including ganglion cells and photoreceptors are favored 
      over hRPCs differentiation due to hUCMSCCM or hADSCCM. Under FBS induced 
      differentiation conditions, hRPCs treated with hUCMSCCM or hADSCCM increase the 
      expression of retinal neuron and photoreceptor specific markers. These results 
      suggest that hUCMSCCM and hADSCCM can stimulate the hRPC proliferation, promote 
      its adherence and support hRPC neuronal and photoreceptor differentiation. These 
      findings may provide a new strategy to improve the viability of hRPCs and 
      photoreceptor differentiation capacities.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Zhang, Mingqi
AU  - Zhang M
AD  - Clinical Research Center, HE Eye Hospital of HE University, Shenyang, Liaoning 
      Province, People's Republic of China.
FAU - Zhang, Fenglei
AU  - Zhang F
AD  - College of Basic Medicine, HE University, Shenyang, Liaoning Province, People's 
      Republic of China.
FAU - Sun, Jin
AU  - Sun J
AD  - College of Basic Medicine, HE University, Shenyang, Liaoning Province, People's 
      Republic of China.
FAU - Sun, Yan
AU  - Sun Y
AD  - Clinical Research Center, HE Eye Hospital of HE University, Shenyang, Liaoning 
      Province, People's Republic of China.
FAU - Xu, Ling
AU  - Xu L
AD  - Clinical Research Center, HE Eye Hospital of HE University, Shenyang, Liaoning 
      Province, People's Republic of China.
FAU - Zhang, Donglei
AU  - Zhang D
AD  - College of Basic Medicine, HE University, Shenyang, Liaoning Province, People's 
      Republic of China.
FAU - Wang, Zhuoshi
AU  - Wang Z
AD  - Clinical Research Center, HE Eye Hospital of HE University, Shenyang, Liaoning 
      Province, People's Republic of China. Electronic address: hsyk2017@163.com.
FAU - He, Wei
AU  - He W
AD  - College of Basic Medicine, HE University, Shenyang, Liaoning Province, People's 
      Republic of China. Electronic address: hewei0111@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170731
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Cell Adhesion/*physiology
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation/*physiology
MH  - Cells, Cultured
MH  - *Culture Media, Conditioned
MH  - Humans
MH  - Mesenchymal Stem Cells/*physiology
MH  - Neural Stem Cells/*physiology
MH  - Retinal Neurons/*physiology
MH  - *Tissue Banks
OTO - NOTNLM
OT  - Adhesion
OT  - Adipose derived stem cells
OT  - Condition medium
OT  - Neuronal differentiation
OT  - Proliferation
OT  - Retinal progenitor cells
OT  - Umbilical cord mesenchymal stem cells
EDAT- 2017/08/05 06:00
MHDA- 2018/05/29 06:00
CRDT- 2017/08/05 06:00
PHST- 2017/04/20 00:00 [received]
PHST- 2017/07/29 00:00 [revised]
PHST- 2017/07/29 00:00 [accepted]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2018/05/29 06:00 [medline]
PHST- 2017/08/05 06:00 [entrez]
AID - S0304-3940(17)30626-2 [pii]
AID - 10.1016/j.neulet.2017.07.053 [doi]
PST - ppublish
SO  - Neurosci Lett. 2017 Sep 14;657:62-68. doi: 10.1016/j.neulet.2017.07.053. Epub 
      2017 Jul 31.