PMID- 28774719
OWN - NLM
STAT- MEDLINE
DCOM- 20190215
LR  - 20190215
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Linking)
VI  - 112
DP  - 2018 Jan
TI  - Defective methionine metabolism in the brain after repeated blast exposures might 
      contribute to increased oxidative stress.
PG  - 234-238
LID - S0197-0186(17)30367-4 [pii]
LID - 10.1016/j.neuint.2017.07.014 [doi]
AB  - Blast-induced traumatic brain injury (bTBI) is one of the major disabilities in 
      Service Members returning from recent military operations. The neurobiological 
      underpinnings of bTBI, which are associated with acute and chronic 
      neuropathological and neurobehavioral deficits, are uncertain. Increased 
      oxidative stress in the brain is reported to play a significant role promoting 
      neuronal damage associated with both brain injury and neurodegenerative 
      disorders. In this study, brains of rats exposed to repeated blasts in a shock 
      tube underwent untargeted profiling of primary metabolism by automatic linear 
      exchange/cold injection GC-TOF mass spectrometry and revealed acute and sub-acute 
      disruptions in the metabolism of the essential amino acid methionine and 
      associated antioxidants. Methionine sulfoxide, the oxidized metabolite of 
      methionine, showed a sustained increase in the brain after blast exposure which 
      was associated with a significant decrease in cysteine, the amino acid derived 
      from methionine. Glutathione, the antioxidant synthesized from cysteine, also 
      concomitantly decreased as did the antioxidant ascorbic acid. Reductions in 
      ascorbic acid were accompanied by increased levels of its oxidized metabolite, 
      dehydroascorbic acid and other metabolites such as threonic acid, isothreonic 
      acid, glycolic acid and oxalic acid. Fluorometric analysis of the brains showed 
      acute and sub-acute increase in total reactive oxygen species. In view of the 
      fundamental importance of glutathione in the brain as an antioxidant, including 
      its role in the reduction of dehydroascorbic acid to ascorbic acid, the 
      disruptions in methionine metabolism elicited by blast exposure might prominently 
      contribute to neuronal injury by promoting increased and sustained oxidative 
      stress.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Arun, Peethambaran
AU  - Arun P
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA. Electronic address: peethambaran.arun.ctr@mail.mil.
FAU - Rittase, William B
AU  - Rittase WB
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA.
FAU - Wilder, Donna M
AU  - Wilder DM
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA.
FAU - Wang, Ying
AU  - Wang Y
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA.
FAU - Gist, Irene D
AU  - Gist ID
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA.
FAU - Long, Joseph B
AU  - Long JB
AD  - Blast-Induced Neurotrauma Branch, Center for Military Psychiatry and 
      Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, 
      USA. Electronic address: joseph.b.long.civ@mail.mil.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170731
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Reactive Oxygen Species)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Blast Injuries/*metabolism/pathology
MH  - Brain/*metabolism/pathology
MH  - Brain Injuries/*metabolism/pathology
MH  - Male
MH  - Methionine/*metabolism
MH  - Oxidative Stress/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Antioxidants
OT  - Ascorbic acid
OT  - Blast exposure
OT  - Cysteine
OT  - Glutathione
OT  - Methionine
OT  - Oxidative stress
OT  - Traumatic brain injury
EDAT- 2017/08/05 06:00
MHDA- 2019/02/16 06:00
CRDT- 2017/08/05 06:00
PHST- 2017/06/22 00:00 [received]
PHST- 2017/07/29 00:00 [accepted]
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2019/02/16 06:00 [medline]
PHST- 2017/08/05 06:00 [entrez]
AID - S0197-0186(17)30367-4 [pii]
AID - 10.1016/j.neuint.2017.07.014 [doi]
PST - ppublish
SO  - Neurochem Int. 2018 Jan;112:234-238. doi: 10.1016/j.neuint.2017.07.014. Epub 2017 
      Jul 31.