PMID- 28777137
OWN - NLM
STAT- MEDLINE
DCOM- 20190321
LR  - 20190321
IS  - 1538-943X (Electronic)
IS  - 1058-2916 (Linking)
VI  - 64
IP  - 2
DP  - 2018 Mar/Apr
TI  - Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta 
      Vulgaris.
PG  - 261-269
LID - 10.1097/MAT.0000000000000611 [doi]
AB  - Natural compounds containing polysaccharide ingredients have been employed as 
      candidates for treatment of skin tissue. Herein, for the first time, 
      electrospinning setup was proposed to fabricate an efficient composite 
      nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or 
      Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 
      for skin tissue engineering. Both prepared scaffolds including noncomposite and 
      composite types were studied by Scanning electron microscope (SEM), Fourier 
      transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. 
      Scanning electron microscope examinations have approved the uniform and 
      homogeneous structure of composite nanofibers containing nylon polymer and B. 
      vulgaris extract. FTIR spectroscopy was endorsed the presence of B. vulgaris 
      extract within the interwoven mat of nanofibers. Also, measurement of mechanical 
      property with cell-laden composite scaffolds approved the desirable similarity 
      between corresponding scaffold and native skin tissue. To our surprise, it was 
      found that compared with nylon nanofibrous scaffold, composite sample containing 
      B. vulgaris extract has lower contact angle indicating a higher hydrophilic 
      surface. After cell seeding process of keratinocyte cells on composite and 
      noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 
      diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells 
      onto the corresponding composite electrospun membrane. Epidermal gene expression 
      such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through 
      real-time polymerase chain reaction (PCR) technique. Furthermore, 
      immunocytochemistry results (cytokeratin 10 and loricrin) approved that the 
      original property of keratinocytes was strongly preserved using composite 
      scaffold. The corresponding study tries to introduce a new type of natural-based 
      scaffolds for dermal tissue engineering that exhibits an elastic behavior similar 
      to native skin tissue.
FAU - Ranjbarvan, Parviz
AU  - Ranjbarvan P
FAU - Mahmoudifard, Matin
AU  - Mahmoudifard M
FAU - Kehtari, Mousa
AU  - Kehtari M
FAU - Babaie, Ali
AU  - Babaie A
FAU - Hamedi, Shokoh
AU  - Hamedi S
FAU - Mirzaei, Samaneh
AU  - Mirzaei S
FAU - Soleimani, Masoud
AU  - Soleimani M
FAU - Hosseinzadeh, Simzar
AU  - Hosseinzadeh S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - ASAIO J
JT  - ASAIO journal (American Society for Artificial Internal Organs : 1992)
JID - 9204109
RN  - 0 (Nylons)
SB  - IM
MH  - *Beta vulgaris
MH  - Cell Proliferation
MH  - Humans
MH  - Keratinocytes
MH  - Nanofibers/*chemistry
MH  - *Nylons
MH  - *Skin
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
EDAT- 2017/08/05 06:00
MHDA- 2019/03/22 06:00
CRDT- 2017/08/05 06:00
PHST- 2017/08/05 06:00 [pubmed]
PHST- 2019/03/22 06:00 [medline]
PHST- 2017/08/05 06:00 [entrez]
AID - 10.1097/MAT.0000000000000611 [doi]
PST - ppublish
SO  - ASAIO J. 2018 Mar/Apr;64(2):261-269. doi: 10.1097/MAT.0000000000000611.