PMID- 28778318
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20171226
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 190
DP  - 2017 Aug
TI  - Dendritic cell-targeted porcine reproductive and respiratory syndrome virus 
      (PRRSV) antigens adjuvanted with polyinosinic-polycytidylic acid (poly (I:C)) 
      induced non-protective immune responses against heterologous type 2 PRRSV 
      challenge in pigs.
PG  - 18-25
LID - S0165-2427(17)30079-X [pii]
LID - 10.1016/j.vetimm.2017.07.003 [doi]
AB  - Porcine Reproductive and Respiratory Syndrome (PRRS) is an economically important 
      swine viral disease worldwide. Current modified live-attenuated vaccines are 
      ineffective against heterologous strains of PRRS virus (PRRSV) circulating in the 
      field. In this study, we evaluated three dendritic cell (DC)-targeted vaccine 
      candidates for their protective efficacy against heterologous PRRSV challenge. 
      Ectodomain regions of DNA-shuffled structural proteins GP3, GP4, GP5 and M of 
      PRRSV were fused together to form the vaccine antigen which was in turn fused 
      with one of three recombinant antibodies each specific to a DC receptor: DC-SIGN, 
      Langerin, and DEC205. The recombinant antibody-fused vaccine antigens were 
      co-administered with polyinosinic-polycytidylic acid (poly (I:C)) adjuvant and 
      subsequently challenged with a heterologous type 2 PRRSV strain (NADC20) in pigs. 
      Our results demonstrate that pigs in DC-SIGN- and DEC205-targeted, but not 
      Langerin- and non-targeted, vaccine groups showed significant IFN-gamma- and 
      IL-4-specific CD4T cell immune responses against the vaccine antigen in 7days 
      post-challenge. Pigs in DC-SIGN- and Langerin-targeted vaccine groups showed 
      greatly reduced IgG responses as compared to the DEC205- and non-targeted vaccine 
      groups. The immune responses induced by DC-targeted vaccines did not reduce 
      viremia and lung pathological lesions in type 2 PRRSV-challenged pigs. In 
      contrast, pigs in Langerin-targeted vaccine group showed significantly increased 
      serum viral titers and viral antigen in lung tissues at 7 and 14days 
      post-challenge respectively. In conclusion, specific targeting of PRRSV antigen 
      through DC-SIGN or DEC205 or Langerin-specific antibodies in the presence of poly 
      (I:C) adjuvant induced immune responses that failed to protect pigs against 
      heterologous type 2 PRRSV challenge.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Subramaniam, Sakthivel
AU  - Subramaniam S
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Pineyro, Pablo
AU  - Pineyro P
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 50011, USA.
FAU - Derscheid, Rachel J
AU  - Derscheid RJ
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 50011, USA.
FAU - Madson, Darin M
AU  - Madson DM
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 50011, USA.
FAU - Magstadt, Drew R
AU  - Magstadt DR
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 50011, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA. Electronic address: xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
DEP - 20170710
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Vaccines)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - Dendritic Cells/immunology/virology
MH  - Poly I-C/pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Vaccines/immunology/*pharmacology
OTO - NOTNLM
OT  - Dendritic cell targeting
OT  - Porcine DC-SIGN
OT  - Porcine DEC205
OT  - Porcine langerin
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Subunit vaccine
EDAT- 2017/08/06 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/08/06 06:00
PHST- 2017/02/13 00:00 [received]
PHST- 2017/06/23 00:00 [revised]
PHST- 2017/07/07 00:00 [accepted]
PHST- 2017/08/06 06:00 [entrez]
PHST- 2017/08/06 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
AID - S0165-2427(17)30079-X [pii]
AID - 10.1016/j.vetimm.2017.07.003 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2017 Aug;190:18-25. doi: 10.1016/j.vetimm.2017.07.003. 
      Epub 2017 Jul 10.