PMID- 2877862
OWN - NLM
STAT- MEDLINE
DCOM- 19861224
LR  - 20081121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 119
IP  - 6
DP  - 1986 Dec
TI  - Opioids act centrally to modulate stress-induced decrease in luteinizing hormone 
      in the rat.
PG  - 2445-50
AB  - Because endogenous opioid peptides (EOP) and CRF are activated during stress and 
      decrease LH levels when injected centrally, we have explored the roles of these 
      peptides in the stress-induced inhibition of LH secretion. Three opioid peptide 
      systems [i.e. endorphin (END), enkephalin, dynorphin (DYN)], are present in the 
      hypothalamus, acting on different opiate receptor subtypes (i.e. mu, delta, 
      kappa). We first evaluated which EOP might be involved in the stress-induced 
      decrease of LH levels. Immunoneutralization of EOP and pharmacological blockade 
      of opiate receptors were used to reverse the decrease in LH induced by 
      inescapable intermittent footshock in castrated male rats. Anti-beta-END and 
      anti-DYN-A serum (intracerebroventricularly [icv]) or pretreatment with beta-END 
      antagonist, beta-human END-(6-31) (2 and 5 nmol, icv), or with kappa-antagonists, 
      Mr1452 MS and Mr2266 BS (10 mg/kg BW, ip), reversed electroshock-induced decrease 
      in LH concentrations. beta-funaltrexamine (beta-FNA), an irreversible mu 1-opiate 
      receptor antagonist, was partially effective in blocking the inhibitory effect of 
      stress on LH levels. Neither passive immunization with anti-enkephalin nor the 
      pretreatment with the delta-opiate receptor antagonist, ICI 154,129, (10 and 100 
      nmol, icv) modified the effect of stress. We then evaluated which endogenous 
      opioid ligands and/or receptors might mediate the inhibitory effect on LH levels 
      of 2 nmol ovine CRF injected icv. Anti-beta-END serum and beta-human END-(6-31), 
      reversed the CRF-induced decrease of LH concentrations, whereas beta-FNA was only 
      partially active. Anti-DYN-A and anti-ENK serum, kappa- and delta-antagonists did 
      not prevent the decline of LH levels in rats receiving CRF centrally. These data 
      suggest that stress-induced inhibition of LH secretion involves the stimulation 
      of beta-END and DYN-A systems via mu/epsilon- or kappa-opiate receptors and that 
      the decrease in circulating LH levels induced by centrally administered CRF may 
      be mediated by the activation of beta-END system. Therefore, it is possible that 
      the activation of the central CRF/beta-END pathway may play an important role in 
      the stress-induced inhibition of reproductive functions.
FAU - Petraglia, F
AU  - Petraglia F
FAU - Vale, W
AU  - Vale W
FAU - Rivier, C
AU  - Rivier C
LA  - eng
GR  - AM-26741/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Endorphins)
RN  - 0 (Enkephalins)
RN  - 0 (Receptors, Opioid)
RN  - 60617-12-1 (beta-Endorphin)
RN  - 74913-18-1 (Dynorphins)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Corticotropin-Releasing Hormone/*pharmacology
MH  - Dynorphins/*physiology
MH  - Electroshock
MH  - Endorphins/*physiology
MH  - Enkephalins/*physiology
MH  - Immunologic Techniques
MH  - Luteinizing Hormone/*blood
MH  - Male
MH  - Rats
MH  - Receptors, Opioid/drug effects/*physiology
MH  - Stress, Physiological/*blood
MH  - beta-Endorphin
EDAT- 1986/12/01 00:00
MHDA- 1986/12/01 00:01
CRDT- 1986/12/01 00:00
PHST- 1986/12/01 00:00 [pubmed]
PHST- 1986/12/01 00:01 [medline]
PHST- 1986/12/01 00:00 [entrez]
AID - 10.1210/endo-119-6-2445 [doi]
PST - ppublish
SO  - Endocrinology. 1986 Dec;119(6):2445-50. doi: 10.1210/endo-119-6-2445.