PMID- 2877868
OWN - NLM
STAT- MEDLINE
DCOM- 19870116
LR  - 20190813
IS  - 0013-9580 (Print)
IS  - 0013-9580 (Linking)
VI  - 27
IP  - 6
DP  - 1986 Nov-Dec
TI  - Dicarboxylic amino acids block epileptiform activity in hippocampal slice.
PG  - 678-84
AB  - Effects of prolonged (5-10 min) continuous perfusion of excitatory amino acids on 
      penicillin (PEN)-evoked epileptiform activity in hippocampal slices were examined 
      with extracellular and intracellular recordings. L-glutamate (GLU), L-aspartate 
      (ASP), quisqualate (QUIS), and N-methyl-D,L-aspartate reversibly depressed 
      multiple (epileptiform) population spikes elicited by PEN (1.7 mM). 
      Intracellularly recorded, PEN-evoked paroxysmal depolarization shifts (PDS) were 
      partially blocked by 1 mM GLU and largely eliminated by 2 mM GLU or ASP. In the 
      presence of PEN, perfusion with both GLU and ASP induced a transient 4 to 6-mV 
      depolarization, usually followed by spontaneous return of membrane potential to 
      control levels. During the amino acid (AA)-induced block of epileptiform 
      activity, there was no significant change in resting membrane potential, input 
      resistance, or the ability to fire action potentials in response to 
      depolarization, indicating that the decreased responsiveness is not a consequence 
      of nonspecific pyramidal cell overdepolarization. The observed depression of 
      epileptiform activity by continued exposure to GLU and its analogues may reflect 
      desensitization or another regulatory mechanism to limit overexcitation.
FAU - ffrench-Mullen, J M
AU  - ffrench-Mullen JM
FAU - Murphy, T H
AU  - Murphy TH
FAU - Coyle, J T
AU  - Coyle JT
FAU - Fisher, R S
AU  - Fisher RS
LA  - eng
GR  - K07-NS00697/NS/NINDS NIH HHS/United States
GR  - MH00125/MH/NIMH NIH HHS/United States
GR  - NS 13584/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (Amino Acids)
RN  - 0 (Glutamates)
RN  - 0 (Oxadiazoles)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 8OC22C1B99 (Quisqualic Acid)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Amino Acids/*pharmacology/therapeutic use
MH  - Animals
MH  - Aspartic Acid/analogs & derivatives/pharmacology
MH  - Epilepsy/*drug therapy
MH  - Glutamates/pharmacology
MH  - Glutamic Acid
MH  - Hippocampus/*drug effects
MH  - In Vitro Techniques
MH  - Male
MH  - N-Methylaspartate
MH  - Oxadiazoles/pharmacology
MH  - Quisqualic Acid
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1986/11/01 00:00
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PHST- 1986/11/01 00:00 [pubmed]
PHST- 1986/11/01 00:01 [medline]
PHST- 1986/11/01 00:00 [entrez]
AID - 10.1111/j.1528-1157.1986.tb03595.x [doi]
PST - ppublish
SO  - Epilepsia. 1986 Nov-Dec;27(6):678-84. doi: 10.1111/j.1528-1157.1986.tb03595.x.