PMID- 28779549
OWN - NLM
STAT- MEDLINE
DCOM- 20180621
LR  - 20180913
IS  - 1527-6473 (Electronic)
IS  - 1527-6465 (Linking)
VI  - 23
IP  - 11
DP  - 2017 Nov
TI  - Bile duct regeneration and immune response by passenger lymphocytes signals 
      biliary recovery versus complications after liver transplantation.
PG  - 1422-1432
LID - 10.1002/lt.24836 [doi]
AB  - This study aimed to elucidate the impact of epithelial regenerative responses and 
      immune cell infiltration on biliary complications after liver transplantation. 
      Bile duct (BD) damage after cold storage was quantified by a BD damage score and 
      correlated with patient outcome in 41 patients. Bacterial infiltration was 
      determined by fluorescence in situ hybridization (FISH). BD samples were analyzed 
      by immunohistochemistry for E-cadherin, cytokeratin, CD56, CD14, CD4, CD8, and 
      double-immunofluorescence for cytokine production and by messenger RNA (mRNA) 
      microarray. Increased mRNA levels of adherens junctions (P < 0.01) were detected 
      in damaged BDs from patients without complications compared with damaged BDs from 
      patients with biliary complications. Immunohistochemistry showed increased 
      expression of E-cadherin and cytokeratin in BDs without biliary complications 
      (P = 0.03; P = 0.047). FISH analysis demonstrated translocation of bacteria in 
      BDs. However, mRNA analysis suggested an enhanced immune response in BDs without 
      biliary complications (P < 0.01). Regarding immune cell infiltration, CD4(+) and 
      CD8(+) cells were significantly increased in patients without complications 
      compared with those with complications (P = 0.02; P = 0.01). In conclusion, 
      following BD damage during cold storage, we hypothesize that the functional 
      regenerative capacity of biliary epithelium and enhanced local adaptive immune 
      cell infiltration are crucial for BD recovery. Such molecular immunological BD 
      analyses therefore could help to predict biliary complications in cases of 
      "major" epithelial damage after cold storage.Liver Transplantation 23 1422-1432 
      2017 AASLD.
CI  - (c) 2017 by the American Association for the Study of Liver Diseases.
FAU - Junger, Henrik H
AU  - Junger HH
AD  - Department of Surgery, University Medical Center Regensburg, Regensburg, Germany.
FAU - Schlitt, Hans J
AU  - Schlitt HJ
AD  - Department of Surgery, University Medical Center Regensburg, Regensburg, Germany.
FAU - Geissler, Edward K
AU  - Geissler EK
AD  - Department of Surgery, University Medical Center Regensburg, Regensburg, Germany.
FAU - Fichtner-Feigl, Stefan
AU  - Fichtner-Feigl S
AD  - Department of Surgery, University Medical Center Regensburg, Regensburg, Germany.
AD  - Department of General and Visceral Surgery, University Medical Center Freiburg, 
      Freiburg, Germany.
FAU - Brunner, Stefan M
AU  - Brunner SM
AD  - Department of Surgery, University Medical Center Regensburg, Regensburg, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Liver Transpl
JT  - Liver transplantation : official publication of the American Association for the 
      Study of Liver Diseases and the International Liver Transplantation Society
JID - 100909185
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adaptive Immunity
MH  - Adult
MH  - Allografts/immunology/pathology
MH  - Bile Ducts/microbiology/*physiology
MH  - Biliary Tract Diseases/epidemiology/*immunology
MH  - Cold Ischemia/adverse effects
MH  - Cytokines/metabolism
MH  - End Stage Liver Disease
MH  - Epithelium/physiology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Liver/immunology/pathology
MH  - Liver Transplantation/*adverse effects
MH  - Lymphocytes/*immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Postoperative Complications/epidemiology/*immunology
MH  - RNA, Messenger/metabolism
MH  - Regeneration/*immunology
EDAT- 2017/08/06 06:00
MHDA- 2018/06/22 06:00
CRDT- 2017/08/06 06:00
PHST- 2017/04/04 00:00 [received]
PHST- 2017/06/30 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/06 06:00 [pubmed]
PHST- 2018/06/22 06:00 [medline]
PHST- 2017/08/06 06:00 [entrez]
AID - 10.1002/lt.24836 [doi]
PST - ppublish
SO  - Liver Transpl. 2017 Nov;23(11):1422-1432. doi: 10.1002/lt.24836.