PMID- 28779639
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20180709
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 159
DP  - 2017 Nov 1
TI  - Thermo-responsive polymer brushes on glass plate prepared from a new class of 
      amino acid-derived vinyl monomers and their applications in cell-sheet 
      engineering.
PG  - 39-46
LID - S0927-7765(17)30492-7 [pii]
LID - 10.1016/j.colsurfb.2017.07.068 [doi]
AB  - In this study, we present a novel thermo-responsive polymer platform that is 
      based on the alanine methyl ester-containing homopolymer (PNAAMe) and the 
      copolymer with glycine methyl ester-based vinyl monomer (P(NAAMe-co-NAGMe)) 
      brushes prepared via surface-initiated atom transfer radical polymerization. 
      Water contact angles for these brushes measured at different temperatures reveal 
      that the polymer brushes collapse and dehydrate around 13 degrees C and 25 degrees C (T(T)s), 
      respectively, upon elevating the temperature. At 37 degrees C, seeded fibroblasts 
      (NIH/3T3) adhere to and spread well onto these brush surfaces although the 
      copolymer brush of P(NAAMe-co-NAGMe) depresses the number of adherent cells less 
      than half of that for the homopolymer of PNAAMe after 24h of cell culture due to 
      increment in hydrophilicity. To prepare the cell-sheet, the cells are seeded on 
      both polymer brushes and cultured at 37 degrees C in the presence of serum. After 4days, 
      the cells proliferated confluently on these brush surfaces. Lowering the 
      temperature to 4 degrees C and 20 degrees C below T(T) of each brush led to the cell-sheet 
      detachment as a monolayer form from the polymer brushes accompanying with the 
      switching of surface affinity.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Higashi, Nobuyuki
AU  - Higashi N
AD  - Department of Molecular Chemistry and Biochemistry, Faculty of Science and 
      Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. Electronic 
      address: nhigashi@mail.doshisha.ac.jp.
FAU - Hirata, Ayaha
AU  - Hirata A
AD  - Department of Molecular Chemistry and Biochemistry, Faculty of Science and 
      Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
FAU - Nishimura, Shin-Nosuke
AU  - Nishimura SN
AD  - Department of Molecular Chemistry and Biochemistry, Faculty of Science and 
      Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
FAU - Koga, Tomoyuki
AU  - Koga T
AD  - Department of Molecular Chemistry and Biochemistry, Faculty of Science and 
      Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. Electronic 
      address: tkoga@mail.doshisha.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20170727
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Amino Acids)
RN  - 0 (Polymers)
SB  - IM
MH  - Amino Acids/chemistry
MH  - Animals
MH  - Glass/chemistry
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Polymerization
MH  - Polymers/*chemistry
MH  - Surface Properties
MH  - Temperature
OTO - NOTNLM
OT  - Amino acid-derived vinyl polymer
OT  - Cell sheet engineering
OT  - Collapse temperature (T(T))
OT  - Fibroblasts culture
OT  - Surface-initiated atom transfer radical polymerization
OT  - Thermo-responsive polymer brush
EDAT- 2017/08/06 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/08/06 06:00
PHST- 2017/02/13 00:00 [received]
PHST- 2017/07/24 00:00 [revised]
PHST- 2017/07/26 00:00 [accepted]
PHST- 2017/08/06 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2017/08/06 06:00 [entrez]
AID - S0927-7765(17)30492-7 [pii]
AID - 10.1016/j.colsurfb.2017.07.068 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2017 Nov 1;159:39-46. doi: 
      10.1016/j.colsurfb.2017.07.068. Epub 2017 Jul 27.