PMID- 28780069
OWN - NLM
STAT- MEDLINE
DCOM- 20171004
LR  - 20190116
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 88
DP  - 2017 Sep
TI  - Serine protease-related proteins in the malaria mosquito, Anopheles gambiae.
PG  - 48-62
LID - S0965-1748(17)30116-9 [pii]
LID - 10.1016/j.ibmb.2017.07.008 [doi]
AB  - Insect serine proteases (SPs) and serine protease homologs (SPHs) participate in 
      digestion, defense, development, and other physiological processes. In 
      mosquitoes, some clip-domain SPs and SPHs (i.e. CLIPs) have been investigated for 
      possible roles in antiparasitic responses. In a recent test aimed at improving 
      quality of gene models in the Anopheles gambiae genome using RNA-seq data, we 
      observed various discrepancies between gene models in AgamP4.5 and corresponding 
      sequences selected from those modeled by Cufflinks, Trinity and Bridger. Here we 
      report a comparative analysis of the 337 SP-related proteins in A. gambiae by 
      examining their domain structures, sequence diversity, chromosomal locations, and 
      expression patterns. One hundred and ten CLIPs contain 1 to 5 clip domains in 
      addition to their protease domains (PDs) or non-catalytic, protease-like domains 
      (PLDs). They are divided into five subgroups: CLIPAs (22) are clip(1-5)-PLD; 
      CLIPBs (29), CLIPCs (12) and CLIPDs (14) are mainly clip-PD; most CLIPEs (33) 
      have a domain structure of PD/PLD-PLD-clip-PLD(0-1). While expression of the CLIP 
      genes in group-1 is generally low and detected in various tissue- and 
      stage-specific RNA-seq libraries, some putative GPs/GPHs (i.e. single domain gut 
      SPs/SPHs) in group-2 are highly expressed in midgut, whole larva or whole adult 
      libraries. In comparison, 46 SPs, 26 SPHs, and 37 multi-domain SPs/SPHs (i.e. 
      PD/PLD-PLD(>/=1)) in group-3 do not seem to be specifically expressed in digestive 
      tract. There are 16 SPs and 2 SPH containing other types of putative regulatory 
      domains (e.g. LDLa, CUB, Gd). Of the 337 SP and SPH genes, 159 were sorted into 
      46 groups (2-8 members/group) based on similar phylogenetic tree position, 
      chromosomal location, and expression profile. This information and analysis, 
      including improved gene models and protein sequences, constitute a solid 
      foundation for functional analysis of the SP-related proteins in A. gambiae.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Cao, Xiaolong
AU  - Cao X
AD  - Department of Entomology and Plant Pathology, Oklahoma State University, 
      Stillwater, OK 74078, USA.
FAU - Gulati, Mansi
AU  - Gulati M
AD  - Department of Entomology and Plant Pathology, Oklahoma State University, 
      Stillwater, OK 74078, USA.
FAU - Jiang, Haobo
AU  - Jiang H
AD  - Department of Entomology and Plant Pathology, Oklahoma State University, 
      Stillwater, OK 74078, USA. Electronic address: haobo.jiang@okstate.edu.
LA  - eng
GR  - R01 GM058634/GM/NIGMS NIH HHS/United States
GR  - R21 AI112662/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170802
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Insect Proteins)
RN  - EC 3.4.- (Serine Proteases)
SB  - IM
MH  - Animals
MH  - Anopheles/*enzymology/genetics
MH  - Female
MH  - Insect Proteins/genetics/*metabolism
MH  - Insect Vectors/enzymology/genetics
MH  - Male
MH  - Serine Proteases/genetics/*metabolism
PMC - PMC5586530
MID - NIHMS898703
OTO - NOTNLM
OT  - Chromosomal location
OT  - Clip domain
OT  - Expression profiling
OT  - Gene duplication
OT  - Hemolymph protein
OT  - Insect immunity
OT  - Phylogenetic analysis
OT  - Serine protease cascade
EDAT- 2017/08/07 06:00
MHDA- 2017/10/05 06:00
PMCR- 2018/09/01
CRDT- 2017/08/07 06:00
PHST- 2017/05/03 00:00 [received]
PHST- 2017/07/25 00:00 [revised]
PHST- 2017/07/29 00:00 [accepted]
PHST- 2017/08/07 06:00 [pubmed]
PHST- 2017/10/05 06:00 [medline]
PHST- 2017/08/07 06:00 [entrez]
PHST- 2018/09/01 00:00 [pmc-release]
AID - S0965-1748(17)30116-9 [pii]
AID - 10.1016/j.ibmb.2017.07.008 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2017 Sep;88:48-62. doi: 10.1016/j.ibmb.2017.07.008. Epub 
      2017 Aug 2.