PMID- 28782273 OWN - NLM STAT- MEDLINE DCOM- 20191024 LR - 20191024 IS - 1365-2982 (Electronic) IS - 1350-1925 (Linking) VI - 30 IP - 2 DP - 2018 Feb TI - Up-regulation of transient receptor potential vanilloid (TRPV) and down-regulation of brain-derived neurotrophic factor (BDNF) expression in patients with functional dyspepsia (FD). LID - 10.1111/nmo.13176 [doi] AB - BACKGROUND: The role of immune activation in Functional Dyspepsia (FD) patients without previous infection is unclear. We compare the gastric and circulating brain-derived neurotropic factor (BDNF), receptor potential vanilloid type (TRPV) families and various cytokines in FD patients. METHODS: Consecutive adult FD patients (Rome III) with no recent history of gastroenteritis and asymptomatic healthy controls were recruited for upper endoscopy. Subjects with GERD and IBS as predominant symptoms, diabetes mellitus, current or previous Helicobacter pylori infection, psychiatric illness and recent use of NSAID or PPI were excluded. Corpus biopsies and serum samples were collected. KEY RESULTS: Forty three [M:F=8:35, mean age: 35.0 (9.3)] FD patients were compared with 23 healthy controls [M:F=8:15, mean age: 36.6 (10.2)]. FD patients had postprandial distress syndrome (PDS) as predominant sub-type (PDS: 36, EPS: 2). There was no significant difference in the median inflammation score (FD:0 (0-1) vs Control:0 (0-1), P=.79). However, FD patients had significantly higher mRNA expression of TRPV1 (FD:0.014+/-0.007, Control:0.003+/-0.001, 4.6 fold, P=.02) and TRPV2 (FD:0.012+/-0.006, Control:0.003+/-0.001, 4 fold, P=.02) compared to controls. The serum (FD:258.0+/-12.3 ng ml(-1) , Control:319.7+/-18.1 ng ml(-1) , P<.01) and gastric BDNF mRNA (FD:0.06+/-0.008, Control:0.092+/-0.01, 0.65 fold, P=.02)levels significantly lower in FD patients. Secretion of cytokines (IL-4, IL-5, IL-6, IL-8, IL-10, G-CSF, TGF-beta2, MCP-1)was also highly correlated with dyspeptic symptoms in patients with FD. CONCLUSIONS & INFERENCES: Despite lacking gastric mucosal inflammation, up-regulation of TRPV1 and TRPV2, down-regulation of BDNF were observed in FD patients. These suggest that immune alteration may contribute to the pathogenesis of FD without any previous infection. CI - (c) 2017 John Wiley & Sons Ltd. FAU - Cheung, C K Y AU - Cheung CKY AUID- ORCID: 0000-0003-2415-6600 AD - Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China. FAU - Lan, L L AU - Lan LL AD - Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China. FAU - Kyaw, M AU - Kyaw M AD - Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China. FAU - Mak, A D P AU - Mak ADP AD - Department of Psychiatry, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chan, A AU - Chan A AD - Department of Anatomical, Cellular and Pathology, The Chinese University of Hong Kong, Hong Kong, China. FAU - Chan, Y AU - Chan Y AD - Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China. FAU - Wu, J C Y AU - Wu JCY AD - Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China. LA - eng PT - Journal Article DEP - 20170807 PL - England TA - Neurogastroenterol Motil JT - Neurogastroenterology and motility JID - 9432572 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cytokines) RN - 0 (RNA, Messenger) RN - 0 (TRPV Cation Channels) RN - 0 (TRPV1 protein, human) RN - 0 (TRPV2 protein, human) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Adult MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cytokines/metabolism MH - Dyspepsia/complications/*immunology/*metabolism MH - Female MH - Humans MH - Inflammation/complications/immunology/metabolism MH - Male MH - RNA, Messenger/metabolism MH - Social Control, Formal MH - TRPV Cation Channels/*metabolism MH - Up-Regulation OTO - NOTNLM OT - BDNF OT - TGF OT - TRPV OT - dyspepsia OT - low-grade inflammation EDAT- 2017/08/07 06:00 MHDA- 2019/10/28 06:00 CRDT- 2017/08/08 06:00 PHST- 2017/04/20 00:00 [received] PHST- 2017/07/06 00:00 [accepted] PHST- 2017/08/07 06:00 [pubmed] PHST- 2019/10/28 06:00 [medline] PHST- 2017/08/08 06:00 [entrez] AID - 10.1111/nmo.13176 [doi] PST - ppublish SO - Neurogastroenterol Motil. 2018 Feb;30(2). doi: 10.1111/nmo.13176. Epub 2017 Aug 7.