PMID- 28782810
OWN - NLM
STAT- MEDLINE
DCOM- 20180507
LR  - 20180507
IS  - 1440-169X (Electronic)
IS  - 0012-1592 (Linking)
VI  - 59
IP  - 6
DP  - 2017 Aug
TI  - Establishment of estrogen receptor 1 (ESR1)-knockout medaka: ESR1 is dispensable 
      for sexual development and reproduction in medaka, Oryzias latipes.
PG  - 552-561
LID - 10.1111/dgd.12386 [doi]
AB  - Estrogens play fundamental roles in regulating reproductive activities and they 
      act through estrogen receptor (ESR) in all vertebrates. Most vertebrates have two 
      ESR subtypes (ESR1 and ESR2), whereas teleost fish have at least three (Esr1, 
      Esr2a and Esr2b). Intricate functionalization has been suggested among the Esr 
      subtypes, but to date, distinct roles of Esr have been characterized in only a 
      limited number of species. Study of loss-of-function in animal models is a 
      powerful tool for application to understanding vertebrate reproductive biology. 
      In the current study, we established esr1 knockout (KO) medaka using a TALEN 
      approach and examined the effects of Esr1 ablation. Unexpectedly, esr1 KO medaka 
      did not show any significant defects in their gonadal development or in their 
      sexual characteristics. Neither male or female esr1 KO medaka exhibited any 
      significant changes in sexual differentiation or reproductive activity compared 
      with wild type controls. Interestingly, however, estrogen-induced vitellogenin 
      gene expression, an estrogen-responsive biomarker in fish, was limited in the 
      liver of esr1 KO males. Our findings, in contrast to mammals, indicate that Esr1 
      is dispensable for normal development and reproduction in medaka. We thus provide 
      an evidence for estrogen receptor functionalization between mammals and fish. Our 
      findings will also benefit interpretation of studies into the toxicological 
      effects of estrogenic chemicals in fish.
CI  - (c) 2017 Japanese Society of Developmental Biologists.
FAU - Tohyama, Saki
AU  - Tohyama S
AD  - Graduate School of Nutritional and Environmental Sciences, University of 
      Shizuoka, Shizuoka, 422-8526, Japan.
AD  - Graduate School of Nanobioscience, Yokohama City University, Yokohama, Kanagawa, 
      236-0027, Japan.
FAU - Ogino, Yukiko
AU  - Ogino Y
AD  - Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
FAU - Lange, Anke
AU  - Lange A
AD  - Biosciences, College of Life and Environmental Sciences, University of Exeter, 
      Exeter, EX4 4QD, UK.
FAU - Myosho, Taijun
AU  - Myosho T
AD  - Graduate School of Nutritional and Environmental Sciences, University of 
      Shizuoka, Shizuoka, 422-8526, Japan.
FAU - Kobayashi, Tohru
AU  - Kobayashi T
AD  - Graduate School of Nutritional and Environmental Sciences, University of 
      Shizuoka, Shizuoka, 422-8526, Japan.
FAU - Hirano, Yu
AU  - Hirano Y
AD  - Institute of Advanced Medicine, Wakayama Medical University, Wakayama, 641-8509, 
      Japan.
FAU - Yamada, Gen
AU  - Yamada G
AD  - Institute of Advanced Medicine, Wakayama Medical University, Wakayama, 641-8509, 
      Japan.
FAU - Sato, Tomomi
AU  - Sato T
AD  - Graduate School of Nanobioscience, Yokohama City University, Yokohama, Kanagawa, 
      236-0027, Japan.
FAU - Tatarazako, Norihisa
AU  - Tatarazako N
AD  - Center for Health and Environmental Risk Research, National Institute for 
      Environmental Studies, Tsukuba, Ibaraki, 305-8506, Japan.
FAU - Tyler, Charles R
AU  - Tyler CR
AD  - Biosciences, College of Life and Environmental Sciences, University of Exeter, 
      Exeter, EX4 4QD, UK.
FAU - Iguchi, Taisen
AU  - Iguchi T
AD  - Graduate School of Nanobioscience, Yokohama City University, Yokohama, Kanagawa, 
      236-0027, Japan.
FAU - Miyagawa, Shinichi
AU  - Miyagawa S
AUID- ORCID: 0000-0003-1360-6215
AD  - Institute of Advanced Medicine, Wakayama Medical University, Wakayama, 641-8509, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20170807
PL  - Japan
TA  - Dev Growth Differ
JT  - Development, growth & differentiation
JID - 0356504
RN  - 0 (Environmental Biomarkers)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Environmental Biomarkers/genetics/physiology
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Female
MH  - Fish Proteins/genetics/*metabolism
MH  - Male
MH  - Oryzias/genetics/*physiology
MH  - Reproduction/genetics/*physiology
MH  - Sexual Development/genetics/*physiology
OTO - NOTNLM
OT  - estrogen receptor
OT  - fish reproduction
OT  - medaka
OT  - sex differentiation
EDAT- 2017/08/08 06:00
MHDA- 2018/05/08 06:00
CRDT- 2017/08/08 06:00
PHST- 2017/05/31 00:00 [received]
PHST- 2017/06/14 00:00 [revised]
PHST- 2017/06/14 00:00 [accepted]
PHST- 2017/08/08 06:00 [pubmed]
PHST- 2018/05/08 06:00 [medline]
PHST- 2017/08/08 06:00 [entrez]
AID - 10.1111/dgd.12386 [doi]
PST - ppublish
SO  - Dev Growth Differ. 2017 Aug;59(6):552-561. doi: 10.1111/dgd.12386. Epub 2017 Aug 
      7.