PMID- 2878430
OWN - NLM
STAT- MEDLINE
DCOM- 19870114
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 83
IP  - 23
DP  - 1986 Dec
TI  - Developmental regulation of glutamine synthetase and carbonic anhydrase II in 
      neural retina.
PG  - 9060-4
AB  - Glutamine synthetase (GS) is expressed in the neural retina only in Muller glia 
      cells and is inducible with cortisol. A chicken genomic clone that contains at 
      least part of the coding region for the GS enzyme was used to investigate 
      developmental changes in the level of GS mRNA in embryonic chicken retina. A 
      major GS transcript (approximately equal to 3 kilobases) detected by the probe 
      begins to accumulate sharply on day 15 of embryonic development. When cortisol is 
      prematurely supplied to early embryonic retina, it induces precocious 
      accumulation of GS mRNA and of the GS enzyme. At later ages, these effects of 
      cortisol are significantly greater, which suggests that competence to transcribe 
      or stabilize GS mRNA in response to stimulation with cortisol increases with 
      development. Carbonic anhydrase II (CA-II) is expressed in early retina in all 
      the cells, but it becomes later restricted to Muller glia. Using cloned CA-II 
      cDNA, we detected a high level of CA-II mRNA in early retina, followed by a 
      decline due to arrest of CA-II mRNA accumulation in differentiated neurons. As 
      glia cells mature, CA-II mRNA and the enzyme increase to a new high level. 
      Therefore, changes in CA-II gene expression during retina development reflect 
      differentiation-dependent cell-type-specific control of CA-II mRNA accumulation.
FAU - Vardimon, L
AU  - Vardimon L
FAU - Fox, L E
AU  - Fox LE
FAU - Moscona, A A
AU  - Moscona AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (RNA, Messenger)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Carbonic Anhydrases/genetics/*metabolism
MH  - Chick Embryo
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Glutamate-Ammonia Ligase/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Retina/embryology/*enzymology/growth & development
PMC - PMC387074
EDAT- 1986/12/01 00:00
MHDA- 1986/12/01 00:01
PMCR- 1987/06/01
CRDT- 1986/12/01 00:00
PHST- 1986/12/01 00:00 [pubmed]
PHST- 1986/12/01 00:01 [medline]
PHST- 1986/12/01 00:00 [entrez]
PHST- 1987/06/01 00:00 [pmc-release]
AID - 10.1073/pnas.83.23.9060 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1986 Dec;83(23):9060-4. doi: 10.1073/pnas.83.23.9060.