PMID- 28787009
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Relationships between and formation dynamics of the microbiota of consumers, 
      producers, and the environment in an abalone aquatic system.
PG  - e0182590
LID - 10.1371/journal.pone.0182590 [doi]
LID - e0182590
AB  - An ecosystem is a community comprising living and nonliving components of the 
      environment. Microbes are ubiquitous elements in each of these components. The 
      dynamics of microbiota formation in an ecosystem is important to elucidate, 
      because how the different components of a system exchange microbes, and how the 
      microbes control ecological processes remain unresolved. In this study, an 
      abalone, Haliotis diversicolor, seed-nursing pond was used as a model system. We 
      first examined changes in bacterial communities during the seedling cultivation 
      of this herbivorous juvenile aquatic invertebrate animal. Denaturing gradient gel 
      electrophoresis (DGGE) and pyrosequencing were used to analyze bacterial 
      community dynamics and spatio-temporal interactions of different system 
      components: consumers (abalone), producers (algae or a substrate), and the 
      environment (water). DGGE fingerprints revealed that the developmental stages of 
      abalone influences bacterial communities of both the abalone and substrate. 
      Although the communities in water fluctuated daily, they could be divided into 
      two clusters that coincided with abalone stages, reflecting the transition from 
      larva to juvenile at around day 21. Pyrosequencing showed that the microbiota in 
      the abalone and substrate had more operational taxonomic units in common than 
      that of either with water. The Bray-Curtis similarity index was used to quantify 
      the formation dynamics of microbiota among the various components of the system. 
      The bacterial communities in producers and consumers showed similar changes. 
      These communities were unstable at the beginning and then slowly stabilized over 
      time. The environmental bacterial community was more stable than the bacterial 
      communities in consumers and producers, and may have been the basis for stability 
      in the system. Our research provides insights into the dynamics of microbiota 
      formation in various biotic elements of a system that will contribute to 
      predictive systems modeling.
FAU - Jiang, Jing-Zhe
AU  - Jiang JZ
AUID- ORCID: 0000-0001-5260-7822
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, China.
FAU - Zhao, Wang
AU  - Zhao W
AD  - Tropical Fisheries Research and Development Center, South China Sea Fisheries 
      Research Institute, Chinese Academy of Fishery Sciences, Sanya, China.
FAU - Liu, Guang-Feng
AU  - Liu GF
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, China.
FAU - Wang, Jiang-Yong
AU  - Wang JY
AD  - Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South 
      China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20170807
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - *Bacteria/genetics/isolation & purification
MH  - Gastropoda/*microbiology
MH  - *Microbiota/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis
PMC - PMC5546691
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/08/09 06:00
MHDA- 2017/09/30 06:00
PMCR- 2017/08/07
CRDT- 2017/08/09 06:00
PHST- 2017/05/12 00:00 [received]
PHST- 2017/07/20 00:00 [accepted]
PHST- 2017/08/09 06:00 [entrez]
PHST- 2017/08/09 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
PHST- 2017/08/07 00:00 [pmc-release]
AID - PONE-D-17-18376 [pii]
AID - 10.1371/journal.pone.0182590 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 7;12(8):e0182590. doi: 10.1371/journal.pone.0182590. 
      eCollection 2017.