PMID- 28789398
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 14
IP  - 2
DP  - 2017 Aug
TI  - Moringa oleifera fruit induce apoptosis via reactive oxygen species-dependent 
      activation of mitogen-activated protein kinases in human melanoma A2058 cells.
PG  - 1703-1710
LID - 10.3892/ol.2017.6288 [doi]
AB  - The present study was performed to determine the effect of Moringa oleifera fruit 
      extract on the apoptosis of human melanoma A2058 cells. A2058 cells were treated 
      for 72 h with Moringa oleifera fruit extract at 50-100 microg/ml, and cell viability 
      with apoptotic changes was examined. The involvement of reactive oxygen species 
      (ROS) and mitogen-activated protein kinases (MAPKs) was examined. It was revealed 
      that Moringa oleifera fruit extract significantly inhibited the cell viability 
      and promoted apoptosis of A2058 cells in a concentration-dependent manner. 
      Moringa oleifera fruit extract-treated A2058 cells exhibited increased activities 
      of cleaved caspase-9 and caspase-3. It also caused an enhancement of MAPK 
      phosphorylation and ROS production. The pro-apoptotic activity of Moringa 
      oleifera fruit extract was significantly reversed by pretreatment with the c-Jun 
      N-terminal kinase (JNK) inhibitor SP600125, extracellular-signal-regulated kinase 
      (ERK) inhibitor PD98058 or ROS inhibitor N-acetyl-L-cysteine. Taken together, 
      Moringa oleifera fruit extract is effective in inducing mitochondrial apoptosis 
      of A2058 cells, which is mediated through induction of ROS formation, and JNK and 
      ERK activation. Moringa oleifera fruit extract may thus have therapeutic benefits 
      for human melanoma A2058 cells.
FAU - Guon, Tae Eun
AU  - Guon TE
AD  - Department of Food and Nutrition, College of Natural Sciences, Duksung Women's 
      University, Seoul 01369, Republic of Korea.
FAU - Chung, Ha Sook
AU  - Chung HS
AD  - Department of Food and Nutrition, College of Natural Sciences, Duksung Women's 
      University, Seoul 01369, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20170531
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5529832
OTO - NOTNLM
OT  - A2058 cells
OT  - Moringa oleifera fruit
OT  - apoptosis
OT  - human melanoma cells
OT  - mitogen-activated protein kinases
OT  - oxidative stress
EDAT- 2017/08/10 06:00
MHDA- 2017/08/10 06:01
PMCR- 2017/05/31
CRDT- 2017/08/10 06:00
PHST- 2016/09/24 00:00 [received]
PHST- 2017/03/09 00:00 [accepted]
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2017/08/10 06:01 [medline]
PHST- 2017/05/31 00:00 [pmc-release]
AID - OL-0-0-6288 [pii]
AID - 10.3892/ol.2017.6288 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Aug;14(2):1703-1710. doi: 10.3892/ol.2017.6288. Epub 2017 May 
      31.