PMID- 28789633
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20220321
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Aug 8
TI  - Variations in 5S rDNAs in diploid and tetraploid offspring of red crucian carp x 
      common carp.
PG  - 75
LID - 10.1186/s12863-017-0542-2 [doi]
LID - 75
AB  - BACKGROUND: The allotetraploid hybrid fish (4nAT) that was created in a previous 
      study through an intergeneric cross between red crucian carp (Carassius auratus 
      red var., female symbol) and common carp (Cyprinus carpio L., male symbol) provided an excellent 
      platform to investigate the effect of hybridization and polyploidization on the 
      evolution of 5S rDNA. The 5S rDNAs of paternal common carp were made up of a 
      coding sequence (CDS) and a non-transcribed spacer (NTS) unit, and while the 5S 
      rDNAs of maternal red crucian carp contained a CDS and a NTS unit, they also 
      contained a variable number of interposed regions (IPRs). The CDSs of the 5S 
      rDNAs in both parental fishes were conserved, while their NTS units seemed to 
      have been subjected to rapid evolution. RESULTS: The diploid hybrid 2nF(1) 
      inherited all the types of 5S rDNAs in both progenitors and there were no signs 
      of homeologous recombination in the 5S rDNAs of 2nF(1) by sequencing of PCR 
      products. We obtained two segments of 5S rDNA with a total length of 16,457 bp 
      from allotetraploid offspring 4nAT through bacterial artificial chromosome (BAC) 
      sequencing. Using this sequence together with the 5S rDNA sequences amplified 
      from the genomic DNA of 4nAT, we deduced that the 5S rDNAs of 4nAT might be 
      inherited from the maternal progenitor red crucian carp. Additionally, the IPRs 
      in the 5S rDNAs of 4nAT contained A-repeats and TA-repeats, which was not the 
      case for the IPRs in the 5S rDNAs of 2nF(1). We also detected two signals of a 
      200-bp fragment of 5S rDNA in the chromosomes of parental progenitors and hybrid 
      progenies by fluorescence in situ hybridization (FISH). CONCLUSIONS: We deduced 
      that during the evolution of 5S rDNAs in different ploidy hybrid fishes, 
      interlocus gene conversion events and tandem repeat insertion events might 
      occurred in the process of polyploidization. This study provided new insights 
      into the relationship among the evolution of 5S rDNAs, hybridization and 
      polyploidization, which were significant in clarifying the genome evolution of 
      polyploid fish.
FAU - Ye, Lihai
AU  - Ye L
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, China.
FAU - Zhang, Chun
AU  - Zhang C
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, China.
FAU - Tang, Xiaojun
AU  - Tang X
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, China.
FAU - Chen, Yiyi
AU  - Chen Y
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, China.
FAU - Liu, Shaojun
AU  - Liu S
AUID- ORCID: 0000-0002-2981-5786
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, China. lsj@hunnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170808
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carps/*genetics
MH  - Chromosome Mapping
MH  - *Diploidy
MH  - *Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Sequence Homology
MH  - *Tetraploidy
PMC - PMC5549377
OTO - NOTNLM
OT  - 5S rDNA
OT  - Allotetraploid fish
OT  - Diploid hybrid fish
OT  - Evolution
OT  - Gene conversion
COIS- ETHICS APPROVAL: All experiments were approved by Animal Care Committee of Hunan 
      Normal University and followed guidelines statement of the Administration of 
      Affairs Concerning Animal Experimentation of China. All samples were raised in 
      natural ponds and all dissections were performed under sodium pentobarbital 
      anesthesia, and all efforts were made to minimize suffering. CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2017/08/10 06:00
MHDA- 2017/11/04 06:00
PMCR- 2017/08/08
CRDT- 2017/08/10 06:00
PHST- 2017/02/03 00:00 [received]
PHST- 2017/08/02 00:00 [accepted]
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
PHST- 2017/08/08 00:00 [pmc-release]
AID - 10.1186/s12863-017-0542-2 [pii]
AID - 542 [pii]
AID - 10.1186/s12863-017-0542-2 [doi]
PST - epublish
SO  - BMC Genet. 2017 Aug 8;18(1):75. doi: 10.1186/s12863-017-0542-2.