PMID- 28791037
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 8
DP  - 2017
TI  - Adaptation of the Pivotal-Differential Genome Pattern for the Induction of 
      Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids 
      within Triticeae Tribe.
PG  - 1300
LID - 10.3389/fpls.2017.01300 [doi]
LID - 1300
AB  - A pivotal-differential evolution pattern is when two allopolyploids share a 
      common genome, which is called pivotal, and differ with respect to the other 
      genome or genomes, called differential. This feature induces the intergenomic 
      recombination between chromosomes of differential genomes, which can lead to 
      speciation. Our study is a cytomolecular insight into this mechanism which was 
      adapted for the induction of intergenomic chromosome recombination in hybrids of 
      synthetic amphidiploids Aegilops biuncialis x S. cereale (UUMMRR) and triticale 
      (AABBRR) where R-genome was pivotal. We observed chromosome recombination events 
      which were induced by both: (1) random chromosome fragmentation and 
      non-homologous chromosome end joining at mitosis of root meristem cells and (2) 
      intergenomic chromosome associations at meiosis of pollen mother cells (PMCs) of 
      F(1) hybrids. Reciprocal chromosome translocations were identified in six F(1) 
      plants and 15 plants of F(2) generation using fluorescence in situ hybridization 
      (FISH) with DNA clones (pTa-86, pTa-k374, pTa-465, pTa-535, pTa-k566, and 
      pTa-713). We observed signals of pTa-86, pTa-535, and pTa-k566 probes in several 
      chromosome breakpoints. The comparison of the DNA clone sequences distinguished a 
      number of common motifs, which can be considered as characteristics of chromosome 
      breakpoint loci. Immunodetection of synaptonemal complex proteins and genomic in 
      situ hybridization analysis at meiosis of PMCs of F(1) hybrids showed, that the 
      homologous pairing of pivotal R-genome chromosomes is crucial for the fertility 
      of F(1) hybrids, however, these chromosomes can be also involved in the 
      intergeneric recombination.
FAU - Kwiatek, Michal T
AU  - Kwiatek MT
AD  - Cereal Genomics Team, Department of Genomics, Institute of Plant Genetics, Polish 
      Academy of SciencesPoznan, Poland.
FAU - Majka, Joanna
AU  - Majka J
AD  - Cytogenetics and Molecular Physiology of Plants Team, Department of Environmental 
      Stress Biology, Institute of Plant Genetics, Polish Academy of SciencesPoznan, 
      Poland.
FAU - Majka, Maciej
AU  - Majka M
AD  - Cereal Genomics Team, Department of Genomics, Institute of Plant Genetics, Polish 
      Academy of SciencesPoznan, Poland.
FAU - Belter, Jolanta
AU  - Belter J
AD  - Cereal Genomics Team, Department of Genomics, Institute of Plant Genetics, Polish 
      Academy of SciencesPoznan, Poland.
FAU - Wisniewska, Halina
AU  - Wisniewska H
AD  - Cereal Genomics Team, Department of Genomics, Institute of Plant Genetics, Polish 
      Academy of SciencesPoznan, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170725
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC5524833
OTO - NOTNLM
OT  - allopolyploids
OT  - chromosome recombination
OT  - evolution
OT  - meiosis
OT  - mitosis
OT  - pivotal-differential theory
OT  - triticeae
EDAT- 2017/08/10 06:00
MHDA- 2017/08/10 06:01
PMCR- 2017/01/01
CRDT- 2017/08/10 06:00
PHST- 2017/05/25 00:00 [received]
PHST- 2017/07/10 00:00 [accepted]
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2017/08/10 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2017.01300 [doi]
PST - epublish
SO  - Front Plant Sci. 2017 Jul 25;8:1300. doi: 10.3389/fpls.2017.01300. eCollection 
      2017.