PMID- 28791053
OWN - NLM
STAT- MEDLINE
DCOM- 20180507
LR  - 20231213
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2017
DP  - 2017
TI  - ROS-Mediated Apoptosis and Genotoxicity Induced by Palladium Nanoparticles in 
      Human Skin Malignant Melanoma Cells.
PG  - 8439098
LID - 10.1155/2017/8439098 [doi]
LID - 8439098
AB  - The present work was designed to investigate the effect of palladium 
      nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, 
      induction of apoptosis, cytotoxicity, and DNA damage. Diseases resulting from 
      dermal exposure may have a significant impact on human health. There is a little 
      study that has been reported on the toxic potential of PdNPs on A375. Cytotoxic 
      potential of PdNPs (0, 5, 10, 20, and 40 mug/ml) was measured by tetrazolium 
      bromide (MTT assay) and NRU assay in A375 cells. PdNPs elicited concentration and 
      time-dependent cytotoxicity, and longer exposure period induced more cytotoxicity 
      as measured by MTT and NRU assay. The molecular mechanisms of cytotoxicity 
      through cell cycle arrest and apoptosis were investigated by AO (acridine 
      orange)/EtBr (ethidium bromide) stain and flow cytometry. PdNPs not only inhibit 
      proliferation of A375 cells in a dose- and time-dependent model but also induce 
      apoptosis and cell cycle arrest at G2/M phase (before 12 h) and S phase (after 
      24 h). The induction of oxidative stress in A375 cells treated with above 
      concentration PdNPs for 24 and 48 h increased ROS level; on the other hand, 
      glutathione level was declined. Apoptosis and DNA damage was significantly 
      increased after treatment of PdNPs. Considering all results, PdNPs showed 
      cytotoxicity and genotoxic effect in A375 cells.
FAU - Alarifi, Saud
AU  - Alarifi S
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
FAU - Ali, Daoud
AU  - Ali D
AUID- ORCID: 0000-0002-1045-4984
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
FAU - Alkahtani, Saad
AU  - Alkahtani S
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
FAU - Almeer, Rafa S
AU  - Almeer RS
AD  - Department of Zoology, College of Science, King Saud University, Riyadh, Saudi 
      Arabia.
LA  - eng
PT  - Journal Article
DEP - 20170716
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Reactive Oxygen Species)
RN  - 5TWQ1V240M (Palladium)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - DNA Damage/drug effects
MH  - Dynamic Light Scattering
MH  - Humans
MH  - Melanoma/*metabolism
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Microscopy, Electron, Transmission
MH  - Oxidative Stress/drug effects
MH  - Palladium/*pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Skin Neoplasms/*metabolism
MH  - Melanoma, Cutaneous Malignant
PMC - PMC5534296
EDAT- 2017/08/10 06:00
MHDA- 2018/05/08 06:00
PMCR- 2017/07/16
CRDT- 2017/08/10 06:00
PHST- 2017/03/02 00:00 [received]
PHST- 2017/05/16 00:00 [revised]
PHST- 2017/06/01 00:00 [accepted]
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2018/05/08 06:00 [medline]
PHST- 2017/07/16 00:00 [pmc-release]
AID - 10.1155/2017/8439098 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2017;2017:8439098. doi: 10.1155/2017/8439098. Epub 2017 Jul 
      16.